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Subject: Biochemistry × Author: Dwivedi, Himanshu × Start date: 2004 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Studies on diosgenin production in fenugreek (Trigonella foenum-graecum L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Dwivedi, Himanshu; Agrawal, Sanjeev
    Fenugreek genotypes were evaluated for nutritional, antinutritional , antioxidant activity and diosgenin contents. Pant Ragini and Pusa Early Bunching varieties showed balanced proportion of nutrient and antinutritional contents with high diosgenin contents. The effect of different processing methods viz. soaking, germination, boiling, autoclaving and roasting on nutritional, antinutritional, antioxidant activity and diosgenin contents were studied. Germination process was found to be better as it enhanced antioxidant activity and reduced anti-nutrients. To study the diosgenin content Pant Ragini, a variety developed by G.B. Pant University was selected to study the effect of different elicitor at seedling level as well as field level. The effect of in vitro germination of fenugreek seed for 5 day with different elicitors (copper, cobalt, chitosan and ethrel) on growth and production of diosgenin was studied. The maximum enhancement in diosgenin content i.e. 70% (relative %) was observed when seed was soaked with 11.77 ppm cobalt treatment followed by 51% with 4.77 ppm copper treatment. The foliar application of these elicitors were also studied at field level for seed yield and diosgenin production. The highest seed yield i.e. 3.06 T/ha was recorded when 100 ppm chitosan was sprayed twice. However, highest diosgenin content i.e. 14.77 mg/g seed dry wt. was recorded when 11.77 ppm cobalt was sprayed. The highest diosgenin production i.e. 38 kg/ha can be obtained when 100 ppm chitosan sprayed twice. The production of diosgenin through tissue culture was also initiated. The highest diosgenin content i.e. 2.95 mg/g dry wt. was recorded in presence of 1ppm BAP and 1ppm 2-4 D for 40 days.