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Subject: Biochemistry × Author: Balwant × Start date: 2004 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular typing of Salmonella isolated from Gangetic water
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-01) Balwant; Mishra, D.P.
    The present study was conducted at the Animal Biotechnology Center, Pantnagar to check the level of Salmonella contamination in the river Ganga. Total 500 samples were collected from 10 stations (50 samples from each station) viz; Gangotri, Uttarkashi, Rishikesh, Haridwar, Hastinapur, Garhmukteshwer, Narora, Kanpur Allahabad and Varanasi. These samples were subjected to Salmonella specific ‘his’ gene PCR. Samples were then Biochemically screened and serotyped at National Salmonella Research Centre IVRI, Izatnagar U.P. These isolates were serotyped as S. Abuja, S. Lagos. S. Pontypridd, S. Chinkual, S. Zwickau, S. Goldenberg and S. Oritamerin. DNA from these samples were extracted. ERIC-PCR, REP-PCR and OMPs profiling were conducted for serovar identification and strain differentiation of Salmonella rare serovars. For molecular typing ERIC- PCR, REP- PCR and OMPs analysis were conducted and it was found that REP- PCR was more efficient in strain differentiation. Therefore the data of ERIC PCR and REP PCR and OMPs profiling were used in Combined Molecular Typing and it was found that combined molecular typing was best typing method. Discriminating ability of ERIC PCR, REP PCR, OMPs profiling and Combined Molecular Typing methods were calculated by Simpson’s Index of Diversity. ERIC PCR showed D value of 0.92 and REP PCR gave 0.99 Value and of OMPs profiling it was found to be 0.94, while it was found 1 in Combined Molecular Typing. A Dendrogram was constructed by using Tree Con software by feeding data of Combined Molecular Typing then similarity and dissimilarity of isolates was checked. Twenty rare isolates showed maximum similarity. Therefore it can be concluded that combined molecular typing by using ERIC-PCR, REP-PCR and OMPs profiling gave better identification of Salmonella rare isolates.