Loading...
Theses
Browse
1 results
Search Results
ThesisItem Open Access Experimental evaluation of pyrolysed bio-oils for the development of anti-bacterial agent(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-12) Badoni, Vaibhav; Verma, A.K.Pyrolysis bio-oils from pine needle, sugarcane leaf residue, wheat straw and rice husk was used to assess the anti-bacterial activity of various plant (Xanthomonas axonopodis, xanthomonas campestris, Xanthomonas oryzae, Erwinia tracheiphila and Ralstonia solanacearum where Pseudomonas fluorescens) and animal pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Salmonella enterica). The pine needle bio-oil has about 50% of pehnolic content that may be responsible for the antibacterial activity against plant and animal pathogenic bacteria. The pine needle bio-oil showed the best antibacterial activity results against plant pathogenic bacteria with the maximum zone of inhibition of 23±0.33 mm and animal pathogenic bacteria with zone of inhibition 15.67±0.33 mm at concentration of 1000 μg/ml. Similarly the aqueous phase of pine-needle bio-oil showed the highest antibacterial activity with maximum zone of inhibition 14.33±1.09mm against plant pathogenic bacteria and 13.67±0.17 in animal pathogenic bacteria. Antibacterial activity of bio-oil was better with respect to aqueous phase in both plant and animal pathogenic bacteria. Pine-needle bio-oil was utilized for preparation of antibacterial agent. The antibacterial agent was prepared by using pine-needle bio-oil at 15%w/v, surfactant sodium-lauryl sulphate at 1%w/v, sequestering agent EDTA at 0.1%w/v and solvent ethanol at 30%v/v. The prepared antibacterial agent had a good efficacy against animal pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Salmonella enterica) with ME values 5.08, 5, 4.55 and 4.33 respectively. An inhibition of bacteria was also observed in carrier based test. However, phenol coefficient was 1 for Staphylococcus aureus indicating that the antibacterial agent was as effective as phenol at the same dilutions.
