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Subject: Biochemistry × Author: Arya, Aradhana × End date: 2024 × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Biological activities of essential oil and whole plant extracts of Callicarpa macrophylla Vahl. in relation to its phytochemical composition
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-08) Arya, Aradhana; Arya, Aradhana; Dubey, S.K.; Dubey, S.K.
    The present study gives a detailed explanation of the phytochemical profile of essential oil and different polarity extracts along with the evaluation of the biological activities of the plant Callicarpa macrophylla collected from the hilly regions of Uttarakhand from the elevation of 1394 m. Fourty-five compounds were identified by GC-MS accounting for 78.79% of the total essential oil composition in which β- Selinene (15.24%) was the prominent constituent. The methanolic extract of the whole plant comprised of 26 compounds constituting 79.74% of the total methanolic extract composition. The major compounds in methanolic extract were Trachylobane (31.87%) and Palmitoleic acid (8.26%). Thirty-two compounds were identified in the hexane extract comprising 63.56% of the total composition. The prominent compounds in hexane extract were Tetratetracontane (9.78%) and Pentatriacontane (7.15%). The Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) was estimated in higher quantities in methanolic extract having 51.27±0.61 mg/gm, 49.47±0.34 mg/gm and 27.89±0.24 mg/gm, respectively; as compared to that of hexane extract having 40.58±0.50 mg/gm, 34.42±0.27 mg/gm and 11.42±0.23 mg/gm, respectively. Methanolic extract demonstrated potent antioxidant activity in terms of DPPH radical scavenging and reducing power activity having the IC50 and RP50 values of 91.97±0.33 μg/mL and 56.72±0.67 μg/mL compared to standards Ascorbic acid (57.66±0.40 μg/mL) and Gallic acid (74.36±0.29 μg/mL) respectively. Both methanolic and hexane extract showed better metal chelating activity; with IC50 value of 10.90±1.40 μg/mL and 41.60±1.31 μg/mL, respectively as compared to the standard EDTA 60.18±0.39 μg/mL. The methanolic extract displayed potent anti-inflammatory activity with an IB50 value of 64.09±1.31 μg/mL comparable to that of the standard Diclofenac Sodium (51.69±0.99 μg/mL). Callicarpa macrophylla possesses a strong therapeutic potential and, if explored further, may prove to be a powerful antioxidant, anti-inflammatory, and cost-effective agent compared to synthetically derived and can replace various artificial synthetic agents from pharmaceutical industries.