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Subject: Aquaculture × Author: Singh, Gulgul × Start date: 2020 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Comparative evaluation of Ferrous sulphate and Zinc sulphate nanoparticles on growth, physiology and gene expression of Jayanti rohu, Labeo rohita
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-03) Singh, Gulgul; Chauhan, R.S.
    Nanoparticle tends to show a distinctive property due to nanoscale size, which becomes beneficial in food and medicine. Considering, the study's objective was to understand and compare the effect of dietary incorporation of Ferrous Sulphate nanoparticles and Zinc Sulphate nanoparticles on Jayanti Rohu, Labeo rohita. In the first experiment, Jayanti Rohu, L. rohita with an initial weight of 126.42±2.5 gm were stocked, 15 no. each in the FRP tanks under indoor conditions. Five nanoparticle enriched diets of ferrous sulphate nanoparticle and zinc sulphate nanoparticle each were formulated (10, 20, 30, 40, 50 mg/kg) and one control basal diet. During the study period of 30 days, tolerance limit, growth, haematological and antioxidant parameters were analysed. Water quality parameters were periodically monitored. The results showed, tolerance limit for both the nanoparticles to be 30 mg/kg feed as there were significant differences in the survival, growth and antioxidant parameters of fishes given nanoparticle enriched diets at the rate of 40 mg/kg feed and 50 mg/kg feed (p≤0.05). In second experiment, four nanoparticle enriched diets were formulated as T1 (Control diet), T2 (30 mg ZnSO4 Nanoparticle /kg feed), T3 (30 mg FeSO4 Nanoparticle /kg feed), and T4 (15 mg of FeSO4 and ZnSO4 nanoparticle each /kg feed). In the study of 120 days, survival rates were not affected by the dietary treatments. A significant increase (p≤0.05) in the final weights of T1, T2, T3 compared with the control was recorded. Significant increment in the haematological and serum profile of fish fed with the T1 & T2 diet was also observed. The carcass composition indicated increased levels of protein in treated groups. Iron and zinc content of the muscle of fishes were significantly higher in treated groups (p≤0.05). Significant upregulations in the IGF1, IGF2, GHR, SOD, CAT, GPX and immune-related (HSP70, C3) genes were observed among the treated groups. Histological alterations were seen in the T4 group. Overall improvement in the health status and growth was observed in the fishes fed with nanoparticle enriched diets.