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Subject: Aquaculture × Author: Arya, Priyanka × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Evaluation of dietary supplementation of Ashwagandha (Withania somnifera) root powder on some reproductive and hematological parameters in male broodstock of Jayantirohu (Labeo rohita)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Arya, Priyanka; Chauhan, R.S.
    The present study was carried out to evaluate efficacy of Withania somnifera (Ashwagadha) root powder as a growth promoting, aphrodisiac and immunostimulating agent in the male brooders of Jayanti rohu (Labeo rohita). It is inferred from phytochemical analysis saponin, alkaloids, flavonoids and starch were present in ashwagandha root powder extract. A total of 200 Labeo rohita male brood fishes with average weight 704.455± 0.18g were dispersed randomly into four treatment groups BP1 (control), BP2, BP3 and BP4 @ 50 males/treatment. The ashwagandha root powder was incorporated at three concentrations (1%, 1.5% and 2%) in feed for conducting the experiment. In control diet D1, ashwagandha was not incorporated. BP1 group fishes were fed with D1 diet, BP2 with D2, BP3 with D3 and BP4 with D4 @ 3% body weight per day for 120 days. The values of water quality parameters were within suitable range of tolerance of the stocked fishes. Addition of ashwagandha in feed showed no detrimental effect on health of the fish. Brooders fed with diet D3 achieved significantly (P<0.05) better net weight gain (104.29±25.94 g), SGR (0.152±.03 %/day) and condition factor (1.242±.03). The monthly study revealed that significantly maximum (P<0.05) GSI (3.33±0.25 %) and testosterone level (2.61±0.03 ng/ml) were achieved in the treatment BP3/D3 during the month of July indicating peak breeding period. Similarly, BP3 treatment broodstock showed significantly (P<0.05) higher value of milt volume (3.03±0.21 ml/kg body weight), milt pH (7.31±0.02), sperm motility (88.98±1.07 %), motility duration (92.80±2.96 sec.), spermatocrit (83.40±1.13 %) and sperm concentration (10.03±0.17 no.×109/ ml) as compared to control treatment. Breeding success showed significant variation in fertilization (98.55±.34 %) and hatching percentage (91.20±.82 %) between BP3 and BP1 treatment. The total erythrocyte count (1.62±.02 cell×106 /μl), total leucocyte count (15.48±.06 cell×103 /μl), haemoglobin (7.68±.04 g%), PCV (22.89±.06 %), MCV (141.01±1.84 μm3), MCH (47.34±.47 Pg) and MCHC (34.15±.49 g/dl) showed significant (P<0.05) increment in treatment BP4 (2% ashwagandha inclusion) as compared to other treatments. The total serum protein (3.10±.03 g/dl), albumin (1.05±.02 g/dl), globulin (2.05±.05 g/dl), A/G ratio (0.51±.02) and total immunoglobulin (1.26±.01 g/dl) varied significantly (P<0.05) higher in BP4 as compared to control and other treatments, thus showing better haematological and serological profile in treated fishes over control. Thus, the obtained results indicate that the root powder of ashwagandha has prime importance in improving reproductive performance and immunological status in male Labeo rohita broodstock if included in fish feed @1.5-2.0%.