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  • ThesisItemOpen Access
    Effect of supplementation of eucalyptus (Eucalyptus globulus) leaf powder in diet containing Phytase enzyme on performance of commercial laying hens
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Manveen Kaur; Ripusudan Kumar
    A feeding trial was conducted to discern the effect of dietary incorporation of eucalyptus (Eucalyptus globulus) leaf powder on growth performance, nutrient utilization, egg quality, haematobiochemical parameters and economics of egg production of White Leghorn laying hens. A total of 72, twenty-eight weeks old laying hens were divided randomly into four treatment groups with 18 hens per treatment having three replicates of 6 birds each. Four treatment groups were, control (T1): basal diet; Treatment (T2):incorporation of 0.3% eucalyptus leaf powder in basal diet containing phytase; Treatment (T3): incorporation of 0.45 % eucalyptus leaf powder in basal diet containing phytase: Treatment (T4): incorporation of 0.60 % eucalyptus leaf powder in basal diet containing phytase. The laying hens' performance was assessed by recording fortnightly average body weight, weekly average feed intake, daily egg output, weekly egg weight, feed conversion ratio, and feed cost of eggs produced by laying chickens. The feeding trial lasted for 12 weeks .A metabolism trial was undertaken for a period of 7 days on two hens randomly selected from each replicate to investigate nutritional utilization. At the end of the feeding trial, blood samples were taken for haematobiochemical analysis. Results showed significant (P<0.05) increase on egg production in the treatment group T2compared to control (T1), T3 and T4 groups in laying hens. There was no significant effect on body weight gain, feed intake and feed conversion ratio among various treatment groups. No significant difference was observed in nutrient utilisation in terms of dry matter, crude protein, ether extract and organic matter among treatment groups. The egg shell quality parameters in terms of shape index, shell weight, shell weight percentage did not differ significantly among treatment groups. However, an improvement was observed in all treatment groups in shell thickness when the diet was incorporated with eucalyptus leaf powder. The egg albumen and yolk traits showed no statistical differences among different treatment groups on adding eucalyptus leaf powder in diet containing phytase. The haematological values i.e. haemoglobin, total erythrocyte (TEC) and total leucocyte count (TLC) in the blood showed significant improvement in T3 and T4as compared to control and T2 treatment group but no statistical differences were noted in packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. There was no significant differences in total protein, albumin, globulin ,calcium ,phosphorus as well as serum ALT and AST activities among the treatment groups. But there was considerable decrease in level of serum cholesterol and triglycerides with increasing level of eucalyptus leaf powder in basal diet containing phytase. Similarly, glucose concentration differed significantly among treatment groups and noted lowest in group fed 0.45 percent eucalyptus leaf powder in basal diet as compared to other treatment group. From the present study ,it is concluded that adding eucalyptus leaf powder in diet can reduce serum cholesterol ,triglycerides and glucose and increase the haemoglobin content, red blood cell count as well as white blood cell count. Hence, incorporation of 0.3 % eucalyptus leaf powder in diet containing phytase ameliorate egg production and is cost effective for improving production performance in White Leghorn laying hens.