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20211
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Subject: Agricultural Chemicals × Author: Himani × End date: 2021 × Start date: 2021 × Type: Thesis ×
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    ThesisItemOpen Access
    Phytochemical analysis and evaluation of biological activities of essential oil of Nepeta hindostana (Roth.) Hains growing in Kumaun region of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Himani; Ravendra Kumar
    The aim of present study was to analyze the chemical composition and to evaluate the biological activities of the essential oil of aerial and root part of N. hindostana (B. Heyne ex Roth) Haines. The plant material was collected from Tarai region of Uttarakhand. Essential oil from the aerial and the root part of N. hindostana (NHAO and NHRO) was extracted by using hydro-distilllation method (Clevenger type apparatus) and the yield (v/w) was 0.3±0.01% and 0.2±0.01% respectively. The essential oils were chemically analysed by GC and GC/MS. Biological activities of NHAO and NHRO such as antifeedant, antioxidant, anti-inflammatory and antidiabetic were also performed using standard protocols. The major compounds present in NHAO and NHRO were mcamphorene (17.7%) and methyl linoleate (13.2%) respectively. Insect antifeedant activity of NHAO and NHRO was done with non-preferential leaf dip method against Spilosoma obliqua. Both the oils possessed significant insect antifeeding activity, however NHRO was more effective. The in vitro antioxidant activity was performed in terms of DPPH radical scavenging, H2O2 radical scavenging and metal chelating assay. In all three assays NHAO exhibited strong activity than that of NHRO Also in In- vitro anti-inflammatory activity NHAO (IB50=20.46±0.21μg/mL) showed a good in vitro anti-inflammatory effect. Moreover, the antidiabetic activity of oils was done using α-amylase inhibition assay taking acarbose as standard. NHRO (IC50=10.86± 0.45 μg/mL) showed good antidiabetic activity than NHAO (IC50=25.33±0.24 μg/mL).