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Theses

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2005 - 200912010 - 20163
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Subject: Agricultural Biotechnology × End date: 2016 × Type: Thesis × Thesis Type: M.Sc ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    Studies on cytopathogenicity of egg drop syndrome-76 virus
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-08) Srivastava, Gaurangi; Rao, V.D.P.
    In the present investigation, an attempt was made to study the cytopathogenicity of Egg drop Syndrome-76 (EDS-76) virus. In this study, VN1 isolate of EDS-76 virus was cultivated in 9-10 days old duck embryo. When infected allantoic fluid was given to laying birds as an antigen, affected birds appeared dull and depressed with diarrhea. The important characteristic was drop in egg production coinciding with production of soft and thin shelled eggs. The virus was isolated from infected allantoic fluid in primary chicken embryo liver (CEL) cell culture. The cytopathic effects characterized by rounding and degeneration of cells were evident from the first passage itself. May Grunwald and Giemsa stained preparations of infected CEL cells culture revealed eosinophilic intranuclear inclusion bodies with distorted nuclei. AGPT performed using infected duck allantoic fluid and standard and experimentally raised hyperimmune sera against EDS-76 virus, revealed precipitin lines. But CIE was unable to detect the presence of virus in infected duck allantoic fluid and infected cell culture supernatants. FAT performed to detect the presence of EDS-76 virus in infected CEL cell culture exhibit intense intranuclear yellow fluorescence. The haemagglutination property of the virus was studied in infected duck allantoic fluid and infected CEL cell culture. The HI test was performed using hyperimmune serum to confirm the specificity of HA test and a 100% correlation was obtained.
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    ThesisItemOpen Access
    Preparation and characterization of finger millet prolamin based nano-delivery vehicle for lipophilic substances
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2015-07) Subin Raj, C.K.; Pandey, Dinesh
    Bioactives such as omega-fatty acids, phytosterols, vitamins, carotenoids are all known to reduce the risk associated with a number of chronic diseases and help in improving public health. However, these bioactive compounds /drugs are exhibit low bioavailability due to their low permeability, biostability, solubility, limited retention time and poor uptake or absorption in human body when they are consumed through food items such as fruits, vegetables etc. These constraints limit their use in development of functional foods and nutraceuticals. In order to overcome the above mentioned disadvantages, the present study was undertaken in the ”Immunobiologicals & Proteomics lab (Dept. of Molecular Biology & Genetic Engineering)” during the II-semester, 2014-15. This is the first report of a nanodelivery vehicle prepared using finger millet prolamin as the encapsulating matrix over oil in water nanoemulsion of sesame oil containing dissolved β-carotene, which was used as a model bioactive compound. The nanoparticles were prepared in a single step ultrasonication process and did not involve the use of organic solvents and cross-linking agents. Effects of different parameters on prolamin based nanoparticle were investigated. Optimal nanoparticles with regular shape, small size (230- 320 nm), high zeta potential and narrow size distribution were obtained at protein contents upto 2% and oil content upto 4% as characterized by electron microscopy and Zetasizer. The delivery system was shown to have high encapsulation and loading efficiencies. During simulated in vitro digestion, sustained release of β-carotene was observed from the prolamin stabilized nanoemulsion. The cytotoxicity and influence of the nanoparticles was assessed on Caco-2 cells, and the results demonstrated that the nanoparticles were biocompatible and non-toxic to the Caco-2 cells even at concentration as high as 0.5 mg mL-1 . There was observed differentiation in the cells challenged with higher concentrations of the nanoparticles. The use of food grade protein derived from a low agri-input requiring crop, finger millet could provide a platform for value addition, creating a new market for the agri-produce and thereby helping the farming sector. Such products are also beneficial to consumers, providing a better alternative to pharmaceuticals. This delivery oriented food derived nanoparticle system has applications as an oral agent for the controlled release and enhanced absorption of lipophilic bioactives and drugs. It has the potential to be incorporated and developed into various forms for nutraceuticals for general health benefits of the populace.
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    ThesisItemOpen Access
    Evaluation of T2 transgenic Brassica juncea harbouring MAPK3 gene against Alternaria blight and molecular cloning of WRKY7 transcription factor of Brassica juncea
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-06) Hazra, Abhijit; Gohar Taj
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    ThesisItemOpen Access
    Biosynthetic potential and molecular characterization of endophytic fungi for picrosides from Picrorhiza kurroa Royle ex. Benth
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-01) Kannojia, Geetanjali; Gaur, A.K.