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Studies on avian diversity, biology and behaviour along Beas river conservation reserve
(Punjab Agricultural University, Ludhiana, 2021) Sachin Kumar; Kler, Tejdeep Kaur
River Beas was declared as conservation reserve in year 2017 under section 36A of Wildlife Protection Act, 1972 but no study is available on the avian diversity along river. Therefore, present study was conducted from April 2019 to March 2021along Beas conservation reserve to understand the avian diversity in various sub habitats present along the river by selecting three locations i.e. Location I (Talwara: In foot hills of Shiwaliks), Location II (Beas: near urban settlement) and Location III (Harike: near rural settlement). Each location was further divided as crop fields, riparian, shallow water, edge, island and river. Study revealed that river Beas provides shelter to 245 bird species where 228 species were recorded at Location I with 40 exclusive species, 126 at Location II and 202 at Location III with 13 exclusive species. Crop fields had avian diversity of 104 at Location I, 78 at Location II and 94 at Location III. Riparian zone of river harbours 143 at location I, 83 at location II and 117 at location III. Shallow water river zone had 110 at location I, 126 at location II and 58 at location III. During this study a total of 113 species were recorded to exploiting edge sub habitat and 85 species Island sub habitat for various activities while 126 in shallow water sub habitat of riverine system which shows that the edge and island habitats are equally important from avian conservation point of view. Both Island and edge habitat are made of relatively very small area as compare to overall river habitat zone therefore these sub habitats are easy to manage but need attention during conservation planning. Crop fields and riparian habitats support more single species roosters and single group roosters. Riverine habitats (Edge, Shallow water and Islands) supports more mix group roosters. A total of 527 nests were recorded during study period having 193 nests at Talwara, 115 at Beas and 219 at Harike. Nesting of birds was niche area specific where colony nesters were tree species specific including Baya weaver nesting was specific to Date Palm and Cattle Egret nesting was specific to Kikar. Riparian zone of Talwara was wide and had minimum anthropogenic activities therefore harbour maximum of 143 species in riparian zone while Harike had wide shallow water zone therefore maximum of 126 water dependent species as compare to other two locations. Location II had almost lowest values for all diversity indexes under study (Abundance, Richness, Shannon index, Simpson index) in comparison to Location I and III shows the effect of proximity of human habitation on avian diversity. Though the all six sub-habitats understudy were adjoining to each other still a clear distinction of niche area preference was recorded during study. The birds may enter the adjoin niche but overall abundance remains similar for preferred niche for each bird species. The study concludes that significant number of species recorded have specific niche area requirements that are completed by the river’s sub-habitats including feeding, foraging, roosting and nesting therefore conservation plans should be designed in such a way that whole area come under protection as a single unit while maintaining its sub habitat identity.
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Technologies for utilization of hull less barley for high fibre foods
(Punjab Agricultural University, Ludhiana, 2021) Mahamudu Mohamed Sasamalo; Amarjeet Kaur
Barley (Hordeum vulgare) is the fourth significant cereal cultivated for human use next to wheat, rice and maize. The demands of barley production keep on increasing day to day due to its diverse utilities, nutritive value and ever-increasing demand. Keeping these in view Punjab Agricultural University has developed two hull less barley, variety PL 891 and BHS 352. These two hull less barley varieties PL 891 and BHS 352 were procured from Department of Plant Breeding and Genetics and were given treatments viz. germination, pressure cooking, acid and alkali and milled into flour. Milled sample were analyzed for chemical composition, functional and rheological characteristics. Barley flour was stored and estimated for shelf life. The effect of treatments for changes in functional, nutritional and antinutritional was studied. All the treatments showed increase in protein content, crude fiber and dietary fiber ranging from 12.5912.77%, 4.06-4.52% and 17.48-23.8%, respectively. β-glucan content improved after treatments; ranging from 4.02-5.05%. Processing caused 33.7-74.3%, 24.4-81.4 %, 75.7-84.6% and 43.173.1% reduction in antinutritional factors such as tannins, saponins, phytic acid and trypsin inhibitor, respectively. Notably, the mineral bioavailability as well the in-vitro protein digestibility and the antioxidant potential improved with the processing. Food products such as chapatti, flat bread and cookies were prepared by 30% incorporation level. Barley drink was prepared by using 100% roasted barley grains. Among the food products prepared, for chapatti all the treatments were accepted by the panelist with the highest overall acceptability of 8.50 from those prepared from germinated flour, for flat bread and cookies those prepared from alkali treated grains had highest overall acceptability of 8.55 and 8.50, respectively. Between hull less barley varieties, variety PL 891 was found better in preparation of high fiber chapatti, flat bread and cookies and variety BHS 352 was found better for preparation of fiber barley drink.
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Development of lactic acid starter culture based fermented fruit and vegetable juices
(Punjab Agricultural University, Ludhiana, 2021) Modi, Ritika; Sahota, Parampal
The lactic acid fermented fruit and vegetable beverages have been developed through controlled fermentation using ten allochthonous high lactic acid producing (0.612-1.35%), phenotypically and genotypically characterized, homo-lactic- Lactic Acid Bacterial (LAB) strains as starter culture consortium. Value-added secondary metabolite enriched Turmeric, Amla, Black carrots (Kanji), and Black pearl grapes-based functional beverages were developed with improved nutritive value (15-35%) antioxidants, (21-63%) polyphenols, (20-42%) flavonoids and (24%) carotenoids; microbial quality (1010 LAB CFU/ml) and acceptability (8±0.2) with shelf life of 90 days. The optimized bioprocess parameters using Box-Behnken Design in Response Surface Methodology with 5% (v/v) (107 CFU/ml) active starter culture for Turmeric beverage -[turmeric 2%w/v), lemon juice (5%v/v), ginger juice (1.5%v/v); dilution ratio (1:3 with sterilised water); salt (1%w/v)]; Amla beverage -[amla juice: guava juice: ginger juice (1:1:1.5 %v/v); dilution ratio (1:3); salt (0.6%w/v)]; Kanji beverage -[black carrots juice (100ml); salt and rye (1.5%w/v); dilution ratio (1:3)]; Grapes beverage -[grapes juice (100ml), lemon juice (8% v/v); dilution ratio (1:1.5); salt (1.2%w/v)], pasteurized at 82ºC for 10-15 sec and fermentation at 37ºC for 28 hrs. A generic HACCP plan determining critical control points on the line was recommended as a food safety tool during the preparation of beverages. Unstructured kinetic model so developed depicts maximum LAB growth at 8th hour and highest ∆pH and Vmax on the 28th hour and 8th hour, respectively. The increase in lactic acid production (0.35, 0.55, 0.96, 0.63 %TA), reduction in pH (4.51, 4.96, 3.76, 2.64) with enhanced functional aspects based on total polyphenols (52.30, 45.58, 41.85, 52.29 gallic acid equivalents mg/100ml) and flavonoids (44.20, 31.13, 43.91, 46.96 quercitin equivalents mg/100ml) with significantly stronger scavenging activities for the 2,2diphenyl-1- picrylhydrazyl (DPPH) radical (74.25, 86.36, 86.91, 69.70%) and ferric reducing power (87.9, 94.4, 108.66, 100.3 μM FeSO4 equivalents) was observed for Turmeric, Amla, Kanji and Grapes fermented beverages, respectively. These bio-interventions showed antimicrobial activity against food borne pathogens Staphylococcus aureus MTCC3906, Listeria monocytogenes MTCC657, Klebsiella pneumonia MTCC109, Escherichia coli MTCC443, Aeromonas hydrophila MTCC173, as well arrested the initial phase of MOLT-4 and CaCo2 cancer cells lines, down regulating the expression of proto-oncogenes and up regulating the tumor suppressor gene exhibiting the antitumorigenic effect. Further, hepatoprotective and hypoglycemic effect studies showed administration of functional lactic acid fermented turmeric and amla beverages (10 ml/kg body weight) for 6 weeks, significantly reverse or reduce the physiological, metabolic damage, and histological alterations equivalent to the hepatoprotective drug Liv52 in alcohol-induced liver damaged and hypoglycemic drug glibenclamide in streptozotocin-induced diabetic Wistar rats, respectively. Further, lyophilised freeze-dried turmeric and amla fermented powders were developed which can be consumed as ready-to-use fermented beverages by reconstitution @2% and @3.2% (with sterilized water) with retention of all properties of freshly prepared beverage prepared.
Open Access
Optimizing irigation schedule for wheat (Triticun aestivıum L) through field and simulation studies
(Punjab Agricultural University, 2021) Sukhpreet Singh; Brar, AS
The present study "Optimizing irrigation schedule for wheat (Triticum aestivum L.y" through field and simulation studies" was carried out at Punjab Agricultural University, Ludhiana and Krishi Vigyan Kendra, Ropar during 2017-18 and 2018-19. Experiment I was conducted in split plot design, keeping factorial combinations of three sowing dates 2s" October (D), 10" November (D,) and 25" November (D)}and two cultivars {Unnat PBW 550 (V) and PBW 725 (V)} in main plots and four irrigation schedules {irigation at CuluAo (L.) 50 (1) and PAU recommended irrigation schedule (L4)} in sub plots. The results revealed that J0d decreased by 8.2 and 3.8% from D, to D, and 18.0 and 11.5% from D, to D, during 2017-18 and 110 respectively. The correspondıng figures for Ropar were 6.1 and 1.3% and 17.6 and 12.5% during 017.18 and 2018-19, respectively. The variety PBW 725 produced 9.5 and 11.6% higher yield as compared Unnat PBW 550 during 2017-18 and 2018-19, respectively at Ludhiana and 9.8 and 11.5% at Ropar. respectively. However, the results showed the variety Unnat PBW 550 performed better under late sown conditions as compared to PBW 725. The highest grain yield was obtained in I, which was significantly better than all other depletion-based irigation treatments but statistically at par with L4. The grain yield in I, was 31.4 and 28.5% higher as compared to I; during 2017-18 and 2018-19, respectively whereas at Ropar it was 314 and 43.1%, respectively. The grain yield and ETc in I, and LĻ were at par, but crop and apparent water productivity was significantly higher in I, as compared to CP. The DSSAT-CERES-Wheat model performed well [as revealed by high correlation coefficient (r), low root mean square error (RMSE) and low mean absolute percentage error (MAPE)] in simulating the days to anthesis (r=0.95 and 0.92; RMSE-4.27 and 4.99 days; MAPE-3.60 and 4.20%), maturity (r-0.96 and 0.94; RMSE-6.02 and 7.51 days; MAPE=3.41 and 4.35%), leaf area index (r=0.92 and 0.84; RMSE-0.16 and 0.15; MAPE=4.58 and 4.50%), grain yield (r=0.94 and 0.96; RMSE=133.3 and 214.6 kg ha'; MAPE=2.66 and 4.87%), biological yield (r-0.92 and 0.93, RMSE=130.6 and 397.1 kg ha': MAPE=1.12 and 2.87%), ETc (r-0.95 and 0.91; RMSE=114 and 10.2 mm; MAPE-3.31 and 3.18%) and CWP (-0.88 and 0.85, RMSE-0.07 and 0.09 kg ha'; MAPE-4.50 and 5.60%) during 2017-18 and 2018-19, respectively. Experiment II was also conducted in split plot design, keeping three irigation timings {three irrigations at crown root initiation, booting and milking (CBM); four irigations at crown root initiation, tillering, flowering and milking (CTFM) and five irrigations at crown root initiation, tillering, booting, flowering and dough (CTBFD)} in main plots and factorial combinations of 3 depths of first ITigation {65 mm (F): 75 mm (F.) and 85 mm (F)} and 3 depths of subsequent irigations {55 mm (S): 65 mm (S.) and 75 mm (S)} in sub plots. There was significant increase in grain yield, apparent and crop water productivity when number of irigations were increased from 3 in CBM to 5 in CTBFD. The grain yield increased significantly when depth of first irrigation was increased from 65 to 75 mm whereas further increase Tesulted in numeric increase only. Similarly, the effect of depth of subsequent irigations was significant only p tO 0 mm. The grain yield was maximum in the treatment CTBFD with 75 mm depth of first irrigation but S Was Statistically at par with the treatment CTBFD with 55 and 65 mm irrigation depth of first irrigation. e DSSAT-CERES-Wheat model performed well in simulating the days to anthesis (RMSE-1.19 and 1.94 days; 1MAPE-0.94 and 1.799%), maturity (RMSE=1.59 and 1.86 days: MAPE=0.90 and 1.02%), leaf area index na 0.86; RMSE-0.39 and 0.,13: MAPE=2.33 and 3.43%), grain yield (r=0.94 and 0.98; RMSE=160.6, Kg ha ; MAPE=3.43 and 3.51%), biological yield (r-0.93 and 0.95; RMSE494.0 and 362.7 kg ha PEF3.63 and 2.86%). ETc (r=0 94 and 0.96: RMSE=8.28 and 11.73 mm; MAPE-2.34 and 3.49%o) and TesU.04 and 0.79; RMSE-0.12 and 0.14 mm: MAPE=8.03 and 9.18%) during 2017-18 and 2018-19, respectively. The study finally concluded that simulation modelling along with field experimentation may help in determining , optimum sowing time of different cultivars and optimizing irigation water use in wheat.
Open Access
Millets; antioxidant activity; functional properties; in vitro digestibility; fabricated grains; pasta
(Punjab Agricultural University, Ludhiana, 2021) Sharma, Rajan; Sharma, Savita
The term ‘nutri-cereals’ has been dedicated to ten cereal grains by Government of India owing to their agrarian and nutritional excellence. These are sustainable crops due to lower water requirement and adaption to harsh environmental conditions. These grains possess biologically active compounds which have several health beneficial properties due to antioxidative mechanisms. The present investigation was carried out with the objectives of characterization, processing and value addition of proso millet, foxtail millet, barnyard millet, white and red sorghum. The nutri-cereal grains were characterized for physical and biochemical properties. Further, these grains were subjected to different processing treatments viz. soaking (12 h at 25°C), germination (48 h at 25°C), fermentation (20 h at 38°C) and dual processing (germination and fermentation) at preliminary optimized conditions. Influence of aforesaid processing treatments was assessed on anti-nutritional compounds, bioactive constituents &anti-oxidant potential, techno-functional properties, in vitro starch & protein digestibility and structural-molecular features of nutri-cereal flours. There was significant reduction (p<0.05) in the anti-nutritional factors expect for saponins which increased during germination due to the fact that only those compounds which are plant reserve material are depleted while those involved in defense mechanism are increased during seed germination. Further, increase in total phenolic and flavonoid content corresponded to antioxidant activity which was mainly due to enzymatic degradation of cell wall constituents augmenting the release of bound polyphenols and their biosynthesis during given treatments. Similar changes were also observed in technological properties including water solubility, oil absorption, emulsification potential which increased during these treatments while water absorption, gel consistency and swelling power were affected due to molecular degradation of starch which limited their potential to retain water. Most predominant impact was observed for dual processed nutri-cereal flours in case of all bio-technofunctional parameters. Macromolecular degradation during germination and fermentation was evident from scanning electron micrographs and FTIR spectrum further revealed variation in molecular arrangement of starch and protein fractions. In vitro starch and protein digestibility were also found to increase dur to triggered enzymatic action and better access to substrate after germination and fermentation. Processed nutri-cereals were utilized to develop different food formulations including extruded products (fabricated grains, pasta), fermented product (curd) and indigenous products (premix, pancake, pinni and chappati). It was found that these nutri-cereals flours could be potentially utilized for different products to add nutritional and biological value without significant effect on the product quality and technological functionality. Organoleptic evaluation suggested that processed nutri-cereal products were acceptable with respect to visual, olfactory and textural attributes. It could thus be inferred that nutri-cereals (untreated and processed) are potential ingredients for value added products.
Open Access
Processing and value addition of variety bell peppers into different shelf stable products
(Punjab Agricultural University, Ludhiana, 2021) Navpreet Kaur; Sachdev, Poonam A.
The investigation entitled ‗‗Processing and value addition of variety bell peppers into different shelf stable products‘‘ was undertaken with the objectives to develop technology for the drying of bell peppers, bell pepper pulp and products, frozen bell pepper snacks and to study the organoleptic acceptability as well as shelf stability of the products. In order to get a good quality dried product from bell peppers, the Response Surface Methodology (Box- Behnken Design (BBD) was used for establishing the relationship of response (Antioxidant activity, Total phenols, Moisture content, Rehydration ratio and Coefficient of rehydration) with respect to two independent variables i.e time and temperature for all three peppers i.e. green, red and yellow. Drying at 55°C for15.5 h in a cabinet dryer yielded high quality dried bell pepper. The freeze drying method was found the best to retain physicochemical and phytochemical constituents. The antioxidant activity of freeze dried green, red and yellow bell pepper slices were 40.00 mg TE/100g, 77.33 mg TE/100g and 78.97 mg TE/100g, respectively at the end of storage period. The bell pepper pulp was prepared by three methods i.e cold pulping (CP), hot pulping (HP) and barbecue (B) and evaluated for physicochemical, phytochemical and antioxidant activity. The hot pulping method was found to be the best in retention of phytochemical constituents while antioxidant activity was found highest in cold pulped bell pepper. The formulation of bell pepper sauces was done from red and yellow bell peppers using three different preparation methods (cold pulping (CP), hot pulping (HP) and barbecue (B). The FTIR technique revealed the presence of several compounds (associated with the absorption bands) in the bell pepper sauces whereas other aromatic chemical compounds were estimated qualitatively using gas chromatography-mass spectrophotometry. The hot pulping method of red bell pepper sauce preparation had more enhancing effect on the phytochemical and antioxidant activity. While the sensorial characteristics were better for both red and yellow bell pepper sauce prepared with barbecue method. Utilization of bell pepper powder (red, green and yellow) was also done for making bhujia and pasta. Storage studies (at 25-30 °C/50-87% RH) revealed that the physicochemical parameters of bhujia remained unaffected except moisture which increased significantly (P<0.05) whereas, a significant (P<0.05) reduction in bioactive compounds and antioxidant activity was observed. However, free fatty acid and peroxide values significantly (P<0.05) increased which remained well within maximum permissible limits. The enriched bhujia were microbiologically safe with shelf life of 3 months without use of any chemical preservative. The Bhujia prepared by utilizing dried red, green and yellow powder was highly acceptable in terms of all the sensory attributes. The pasta prepared with 10% bell pepper powder had optimum quality and more acceptability than the control sample. For the preparation of frozen products from bell pepper, two formulations namely F1 with corn flour and rice flour, F2 with gram flour and rice flour were selected. Based on preliminary sensory trials, F2- gram flour+rice flour were selected which yielded better sensory product. The mean ascorbic acid, total phenols and antioxidant activities of frozen snacks was found to be 81.22 mg/100g, 133.43 mg GAE/100g and 45.33%, respectively from end storage studies at -20 °C and revealed that all the physicochemical and phytochemical parameters remained unchanged. Similarly, rancidity parameters increased nonsignificantly (p<0.05). The frozen snacks remained highly acceptable during storage without significant change in quality. The present study has revealed the utility of bell pepper for value addition in various products i.e dried slices, pulp, sauce, frozen snacks, bhujia and pasta.
Open Access
Optimizing Lateral Depth and Spacing for Subsurface Drip Irrigated Rice-Wheat Cropping System under Conservation Agriculture Through Simulation of Water and Nitrate Movement
(Punjab Agricultural University, Ludhiana, 2021) Bajpai, Arpna; Kaushal, Arun
The research study was conducted at Borlaug Institute for South Asia, Ludhiana and Punjab Agricultural University Ludhiana, Punjab to optimize lateral depth and spacing for subsurface drip irrigated rice-wheat cropping system under conservation agriculture through simulation of water and nitrate movement during 2017-20. Experiments were laid out in randomized block design with eight treatments, (six drip irrigated (T1-T6), which were combination of lateral spacing X emitter spacing X dripline buried depth) and two control treatments (T7-T8) with three replication i.e. T1 (67.5 X 30 X 0 cm), T2 (45 X 40 X 0 cm), T3 (67.5 X 30 X 15 cm), T4 (45 X 40 X 15 cm), T5 (67.5 X 30 X 20 cm), T6 (45 X 40 X 20 cm), T7 (conventional flood irrigation method) and T8 (flood irrigation method with conservation agriculture). Grain yield and water productivity were maximum in treatment T4 (Wheat, 6.513 t/ha, 3.832 kg/m3 and Rice, 8.178 t/ha, 1.203 kg/m3 ) and minimum in T7 (Wheat, 5.620 t/ha, 1.406 kg/m3 and Rice, 7.410 t/ha, 0.492 kg/m3 ). Statistically T4 treatment was significantly at par with T6 treatment and higher than all other treatments for both rice and wheat crops. Most uniform soil moisture and nitrate movement were observed under T4. HYDRUS-2D model showed successful performance with Nash-sutcliffe model efficiency coefficient of 0.885 and coefficient of determination as 0.906. Economic analysis showed that treatment T4 (B:C-3.286) was economically viable and significantly higher than treatment T7 (B:C-3.157) only with 95% of drip irrigation subsidy.
Open Access
Optimization of nutritional & biochemical composition and development of health foods - A study of microgreens
(Punjab Agricultural University, Ludhiana, 2021) Bhatt, Pooja; Sharma, Sonika
Microgreens (seedling of vegetable and herbs) are gaining popularity as a new culinary ingredient due to their high nutrient content. The present study optimizes the best combination of cultivation and harvesting to attain nutrient quality in four culinary microgreens belonging to different families. Results revealed that the best technique for optimization of nutrient composition was soaking the seeds overnight, outdoor cultivation and harvesting at an early stage (10th day). Based on NQS 7.1 broccoli microgreens were most nutrient dense microgreen followed by spinach microgreens. Microgreens contain significantly (p<0.05) higher nutrients and bioactive compounds as compared to their sprouts and mature counterparts. Just 100g of microgreens fulfilled more than 100% of Estimated Average Requirements (EAR) of minerals among Indian adults. Drying methods influenced the nutritional composition of the broccoli microgreen powder. β-carotene content (400.54ug/100g), total phenolic content (2645.88 mg GAE/100g) and flavonoids (673.82 mg QE/100g) of shade dried microgreens was significantly higher. A significant higher content of ascorbic acid (16.80 mg/100g), chlorophyll (3.63g/100g), antioxidant activity (67.55%) and all the minerals was reported in microwave dried microgreens. Further fresh microgreens,microgreen juice and powdered microgreens were incorporated in commonly consumed health foods in order to enhance their taste and nutritional composition. All the health products i.e. missi roti, cooked vegetable and dal, salads, juice blend, flavoured milk and food sprinkler supplemented with broccoli microgreens were organoleptic highly acceptable. In term of fresh broccoli microgreens supplementation, 25% in missi roti, 5 and 7.5% in cooked vegetable and dal, 30 and 40% in russian and tofu salad was highly accepted. Incorporation of fresh broccoli microgreen juice at 30% in juice blend and flavoured milk was highly acceptable. Powdered broccoli microgreens supplemented at 40 % of in regular spice mix was highly accepted. Overall nutritional composition in broccoli microgreens supplemented health foods had significantly (p<0.05) high content of vitamins C, β-carotene content and minerals namely calcium, zinc, iron, potassium and magnesium when compared to the control product.
Open Access
Pathological and molecular variation in Puccinia triticina the incitant of leaf/brown rust of wheat and host resistance
(Punjab Agricultural University, Ludhiana, 2021) Harmandeep Kaur; Jaspal Kaur
Leaf/brown rust of wheat, caused by Puccinia triticina (Pt), is one of the most important diseases of wheat worldwide. To study the virulence pattern/ diversity of P. triticina, the surveys for leaf rust occurrence were conducted in Punjab during 2018-2021. SBS Nagar (Saroya block) Ludhiana west, Rupnagar (Anandpur sahib) and Gurdaspur were observed as hot spot areas for leaf rust of wheat and disease severity recorded was in the range of 5-40S at different locations. From virulence profiling of the leaf rust infected samples collected from Punjab, it was found that Pt pathotypes 77-5, 77-9, 77-13 (121R60-1,7 ) and 1R 31 are known to occur in Punjab with varied frequencies. Out of which the prevalence of pathotype 77-9 was maximum (62.85%) followed by 121R60-1,7(31.42%). The genetic diversity among Pt isolates was studied using SSR markers which demonstrated that the new pathotype (121R60-1, 7) is more closely related to the pathotype77-9. To know the effectiveness of Lr genes, the leaf rust differentials (Indian and in Thatcher background) and commercial wheat cultivars/ known Lr gene lines were scored against P. triticina infection at four different locations of Punjab (Ludhiana, Gurdaspur, SBS Nagar & Abohar). The lines carrying Lr genes; Lr 9, Lr 19, Lr 24, Lr 25, Lr 28, Lr 29, Lr 32, Lr 42, Lr 45, Lr 47, Lr 52, Lr 57, Lr 58, Lr 76 exhibited resistance against the most prevalent pathotypes (77-5, 77-9 and 121R60-1, 7) of P. triticina in Punjab. One hundread and ninty seven wheat germplasm lines were evaluated at the seedling stage as well as at the adult plant stage against these pathotypes. Based on the seedling reaction test and adult plant response at Ludhiana and Gurdaspur during 2018-2021, it was inferred that thirty six lines showed highly resistant reaction whereas twenty six lines were highly susceptible with AUDPC value >400 and FRS value >40S. DNA of all these test lines was amplified with Lr gene specific markers to identify the gene(s) present in these lines. Based on the molecular data and race specific infection response at seedling as well as at adult plant stage; the leaf rust resistance genes Lr 9, Lr19, Lr24, Lr25, Lr 28, Lr29, Lr32, Lr42, Lr45, Lr47, Lr52, Lr57, Lr58 and Lr76 were postulated in these lines. All stage resistance was imparted by the genes; Lr9, Lr19, Lr24 and Lr28 against prevalent Pt pathotypes in Punjab. The F3 and F5 generation of seven populations were evaluated against P. triticina from 2018-2021 under field conditions at PAU (Ludhiana). The genotypic ratio 1:2:1 and 1:1 for F3 and F5 population respectively fit significantly on the phenotypic data. For gene mapping the resistant and susceptible bulks along with resistant and susceptible parents were genotyped with 90K SNP chip Ilumina array. The SNPs located on the chromosome 5A for population (IC 321853 X HD 3086); 7B for population (IC 549520 X HD 3086); 1B,1D and 6A for population (IC 252609 X HD 3086); 1A, 1D, 3B, 4B and 5A for population (IC 252742 X HD 3086) and 1A, 1B, 2B, 3B and 7A for population (IC 539316 X HD 3086) were selected for designing the KASP markers.

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