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  • ThesisItemRestricted
    Arthropods diversity under paired row direct seeded rice and management of its major insect pests with biopesticides
    (Punjab Agricultural University, Ludhiana, 2019) Navjot Kaur; Randhawa, Harpal Singh
    The present studies were conducted at Rice Research Area, Punjab Agricultural University, Ludhiana and PAU, Regional Research Station (RRS), Gurdaspur during kharif 2017 to determine arthropods diversity under paired row direct seeded rice and bio efficacy of biopesticides against major insect-pests of rice. The experiment on arthropods diversity was laid out with three dates of sowing and sprayed/unsprayed conditions. The Overall highest arthropods diversity was recorded with second date (first fortnight of June) followed by third (second fortnight of June) and first (second fortnight of May) date of sowing. The higher arthropods diversity was recorded at Gurdaspur as compared to Ludhiana. The maximum incidence of leaffolder and stem borers and population of planthoppers was observed in third (second fortnight of June) date of sowing at both the locations. The leaffolder incidence and planthoppers population was higher at Gurdaspur whereas stem borers damage was higher at Ludhiana. The experiment on bio-efficacy of biopesticides was also conducted at both the locations (Ludhiana and Gurdaspur). It was found that the biopesticides i.e. Neem baan (azadirachtin 1%) @ 3 ml/litre of water was more effective against leaffolder, stem borers and planthoppers. The Beauveria bassiana @ 10 ml/litre of water was also found effective against leaffolder and planthoppers whereas Bt (Delfin WG) @ 10 g/litre of water was effective only against stem borers. The highest cost-benefit ratio was recorded in treatment with chlorpyriphos @10 ml/litre of water followed by Neem baan @ 1 & 2 ml/litre of water. Although comparatively less cost-benefit ratio was observed with biopesticides yet these were safer to our environment and natural enemies. Therefore, it is suggested that farmers should prefer to spray biopesticides for management of insect-pests.
  • ThesisItemRestricted
    Impact of electromagnetic radiation emitted from cell phone towers on breeding biology of birds
    (Punjab Agricultural University, Ludhiana, 2019) Navjot Kaur; Vashishat, Nisha
    Birds are one of the most diverse and evolutionary successful groups which occupy almost every habitat on earth. Birds act asbiological indicator of an environment because of their tiny head with thin cranium, non-conducting plumages, high mobility and practice of inhabiting zones having high electromagnetic field. The present study deals with the impact of electromagnetic radiations emitted from cell phone tower on diversity and breeding biology of birds at two locations; location 1- Punjab Agricultural University (PAU), Ludhiana and location 2- village Baude, Moga. Three sites each with cell phone tower in the centre were selected at two locations and one site without any cell phone tower was selected as control site. The bird diversity was recorded within 200 m radius of cell phone tower and artificial nest boxes were installed to study the breeding biology of birds at selected sites. The maximum bird diversity was recorded at site II (17 bird species) of location 1 than the control site (12 bird species). In spite of the presence of cell phone tower there was significant difference amongst the bird diversity at site II and other selected sites under study which showed minimal effect of electromagnetic radiations on the avian diversity.This may be because the birds observed during the present study were not restricted to this area only and therefore were not under the continuous exposure of electromagnetic radiations. Common Myna occupied artificial nests at all the four selected sites under location 1 (PAU). Common Myna and House Sparrow occupied the artificial nest boxes for breeding at selected sites of location 2. The breeding success of Common Myna was ranging from 50-100% at control sites and 50-75% at sites having cell phone towers. House sparrows occupied 10-40% nests at sites of village Baude but further egg laying was not seen in these occupied nests. This may be attributed to the impact of continuous exposure of these birds to EMR (electromagnetic magnetic radiations) during the breeding season.
  • ThesisItemOpen Access
    AN ECONOMIC ANALYSIS OF CHILD LABOUR IN AGRICULTURAL TOOL MAKING UNITS IN MANDI AHMEDGARH OF SANGRUR DISTRICT OF PUNJAB
    (PAU Ludhiana, 2013) Navjot Kaur; Mini, Goyal
    The present study was conducted to examine the socio – economic and work profile of child labour in agricultural tool making units in Mandi Ahmedgarh of Sangrur district of Punjab. Different factors affecting their employment and the contribution towards their family income was also analyzed. A total number of 31 sickle making units were found in Mandi Ahmedgarh. The primary data were collected from 102 children working in these units. The study revealed that all the children employed in sickle making units were male and their mean age was found to be twelve years. Nearly ninety per cent of the working children were migrants and were from lower social strata of the society. Nearly half of the respondents were illiterate and the parents of majority of working children were also illiterate. The working children were found engaged in the activities like iron moulding, assembling and nailing in the sickle making. Per day mean wages of the child labour were about rupees eighty three only. The main reason which forced the children to join the work force was poverty and lack of their interest in the studies. The working conditions were not good and the behaviour of employer was bad. The employment of children helped the parents to take loan from the employer and gave the freedom to the children to spend their pocket money at their own will. The working children were found indulged in bad habits like smoking, consuming liquor, gutka, gambling etc. Though a social evil, child labour was found helpful in removing the poverty of the family to some extent. The children wanted to be educated and to be successful in their life. The study suggested the provision of night classes for those who wanted to study along with their work so that they may become literate and contribute in the nation’s development.
  • ThesisItemOpen Access
    CHARACTERIZATION OF NARINGINASE ENZYME FOR PRODUCTION OF DEBITTERED LOW ALCOHOLIC NATURALLY CARBONATED KINNOW BEVERAGE
    (PAU Ludhiana, 2013) Navjot Kaur; Param Pal, Sahota
    Naringin, the bitter flavonone glycoside and primary bitter component in citrus fruit juices can be hydrolysed by naringinase enzyme into tasteless component, naringenin. A rapid detection test for naringinase producing microorganisms using FeCl3 has been developed. Naringin an inducer in kinnow juice, mutated yeast Clavispora lusitaniae and induced the production of crude enzyme naringinase with the activity of 0.0135 IU/ml. The parameters optimized for naringinase production were pH (4), temperature (50°C), naringin (0.8%) and rhamnose concentration (0.6%) with high substrate activity Km value of 1.00 mM and Vmax value of 28.56 mM. Divalent cations Cu2+ and Ca2+ competitively inhibited enzyme activity where as Mn2+ and Zn2+ stimulated enzyme activity of 0.024 IU/ml and 0.0079 IU/ml, respectively. The debittering of kinnow juice by Clavispora lusitaniae mutant prompted for utilization of kinnow juice for the production of low alcoholic naturally carbonated fermented kinnow beverage. Technology for the production of low alcoholic naturally carbonated fermented kinnow beverage with yeast Clavispora lusitaniae under optimized fermentation conditions were developed. Microbiological, physiochemical and sensory evaluation of kinnow beverage with 40 percent juice revealed pH 4.2, TSS 10.5°B, acidity 0.53%, ascorbic acid 9.2 mg/100ml, reducing sugar 8.83 percent, total sugars 9.4 percent, limonin 1.7 ppm, naringin 120.8 ppm, β-carotene 0.26μg/L, alcohol 0.28% (w/v), CO2 1.09 bar and plate count 9.3x108 cfu/ml, ranked highest for taste 7.9, aroma 8.5, colour 7.8, astringency 8.25 and overall acceptability 7.8 during storage period of 70 days under refrigerated conditions (4°C). The percentage decrease in limonin and naringin on storage was 54 and 64.8 percent, below the threshold level of limonin (6 ppm) and naringin (600 ppm) respectively. The pasteurization conditions standardized for kinnow beverage were 85°C for 3 sec.
  • ThesisItemOpen Access
    STUDY ON COMBINED EFFECT OF BROMADIOLONE AND CHOLECALCIFEROL (VITAMIN D3) AGAINST HOUSE RAT, Rattus rattus LINNAEUS
    (PAU Ludhiana, 2010) Navjot Kaur; Devinder Kaur, Kocher
    Commensal rodents, especially house rat (Rattus rattus Linnaeus) causes extensive losses by feeding and contaminating the food products and also plays a role in spreading several diseases of health importance. House rats were trapped from various commensal situations and were fed for 5 days in no-choice on standard baits of bromadiolone (0.005%) and cholecalciferol (0.075%) and their combinations having different concentrations mixed in WSO-mix bait. Male and female house rats showed 81.30% and 95.80% average acceptability of standard bait of bromadiolone over plain bait respectively. Hundred per cent mortality of male house rats was observed within 3-6 days, while all female rats died on 4th day of feeding of 0.005% bromadiolone. Both the sexes showed a significant increase in blood clotting time (sec) after 48 hours of feeding as compared to that of 0 hour. The acceptability of standard bait of cholecalciferol (0.075%) over plain bait was 40.10% and 35.30% in male and female house rats, respectively and 100% mortality was observed within 4-10 days in male and 7-14 days in female house rats. Feeding of standard bait of cholecalciferol resulted in stop feeding action in the form of significantly less consumption of this bait from 3rd day onward. Serum calcium level (mg/dL) was found to be significantly high in 0.075% cholecalciferol fed male and female rats after 48 hours of its feeding as compared to 0 hour. R. rattus when fed on four formulated baits having different concentrations of bromadiolone and cholecalciferol i.e. combination-I (0.0025% bromadiolone+0.05% cholecalciferol), combination-II (0.001% bromadiolone+0.05% cholecalciferol), combination-III (0.0025% bromadiolone+0.01% cholecalciferol) and combination-IV (0.001% bromadiolone+0.01% cholecalciferol). Out of these four tested formulated baits, combination-IV (having the lowest concentration of bromadiolone and cholecalciferol) showed efficient rodenticidal potential because of synergistic effect of bromadiolone and cholecalciferol in this combination. It was able to produce 100% mortality and showed a significant delay in blood clotting time (sec) and rise in serum calcium level and was cost effective also. R. rattus L trapped from commensal situations of Ludhiana area showed no resistance towards standard bait of bromadiolone (0.005%) as both male and female house rats (n=60) showed 100% mortality.
  • ThesisItemRestricted
    STUDY ON COMBINED EFFECT OF BROMADIOLONE AND CHOLECALCIFEROL (VITAMIN D3) AGAINST HOUSE RAT, Rattus rattus LINNAEUS
    (2010) Navjot Kaur; Kocher, Devinder Kaur
    by feeding and contaminating the food products and also plays a role in spreading several diseases of health importance. House rats were trapped from various commensal situations and were fed for 5 days in no-choice on standard baits of bromadiolone (0.005%) and cholecalciferol (0.075%) and their combinations having different concentrations mixed in WSO-mix bait. Male and female house rats showed 81.30% and 95.80% average acceptability of standard bait of bromadiolone over plain bait respectively. Hundred per cent mortality of male house rats was observed within 3-6 days, while all female rats died on 4th day of feeding of 0.005% bromadiolone. Both the sexes showed a significant increase in blood clotting time (sec) after 48 hours of feeding as compared to that of 0 hour. The acceptability of standard bait of cholecalciferol (0.075%) over plain bait was 40.10% and 35.30% in male and female house rats, respectively and 100% mortality was observed within 4-10 days in male and 7-14 days in female house rats. Feeding of standard bait of cholecalciferol resulted in stop feeding action in the form of significantly less consumption of this bait from 3rd day onward. Serum calcium level (mg/dL) was found to be significantly high in 0.075% cholecalciferol fed male and female rats after 48 hours of its feeding as compared to 0 hour. R. rattus when fed on four formulated baits having different concentrations of bromadiolone and cholecalciferol i.e. combination-I (0.0025% bromadiolone+0.05% cholecalciferol), combination-II (0.001% bromadiolone+0.05% cholecalciferol), combination-III (0.0025% bromadiolone+0.01% cholecalciferol) and combination-IV (0.001% bromadiolone+0.01% cholecalciferol). Out of these four tested formulated baits, combination-IV (having the lowest concentration of bromadiolone and cholecalciferol) showed efficient rodenticidal potential because of synergistic effect of bromadiolone and cholecalciferol in this combination. It was able to produce 100% mortality and showed a significant delay in blood clotting time (sec) and rise in serum calcium level and was cost effective also. R. rattus L trapped from commensal situations of Ludhiana area showed no resistance towards standard bait of bromadiolone (0.005%) as both male and female house rats (n=60) showed 100% mortality. Keywords: Bromadiolone, cholecalciferol, resistance, synergistic effect, R. rattus ___________________________
  • ThesisItemOpen Access
    Biology of tetranychus urticae koch and seasonal incidence of mites infesting citrus
    (Punjab Agricultural University, Ludhiana, 2014) Navjot Kaur; Bhullar, Manmeet Brar
    The biology of two-spotted spider mite, Tetranychus urticae Koch and seasonal incidence of mites infesting citrus was studied during 2013-2014 at the Acarology Laboratory, Entomological Research Farm, Department of Entomology and New Orchard, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Biological studies on T. urticae revealed that the mean incubation, larval, protonymphal and deutonymphal period was found to be 2.52, 2.74, 2.08, and 2.40 days, respectively. The pre-oviposition, oviposition and post-ovipostion period was 0.96, 1.78 and 6.40 days, respectively. The fecundity of 1.56 eggs/female and longevity of females (9.40 days) and males (6.71 days) was observed. The sex ratio was in favour of females (4.33:1). Studies on oviposition response of T. urticae on different citrus genotypes revealed that oviposition was found to be maximum on SL-4 and G-4 followed by BL-2 and G-4 genotypes and no egg laying was observed on SL-3, SL-7, G-7, BL-1, BL-3 and Tahiti lime. Feeding preference studies revealed that mites preferred KL-7 genotype the most, but had least preference for G-4 genotype. Observations on the seasonal incidence revealed that peak occurrence of Brevipalpus phoenicis was reported during 47th standard week (SW) (23.0 mites/leaf); Tetranychus urticae during 21st SW (6.25 mites/leaf), while Polyphagotarsonemus latus started occurring during November-December (47th to 25th SW). The population of Eutetranychus orientalis was observed during April-June (16th to 26th SW) as with increase in temperature there was increase in population of mites. The population of predatory mites viz. Tydeids, Phytoseiids and Cunaxids was observed throughout the year with peak incidence during 29th SW and 46th SW, respectively. Although the mite populations showed both negative and positive correlations with the various abiotic parameters, but statistically these were found to be non significant.
  • ThesisItemRestricted
    Genetic analysis of important economic traits and validation of molecular markers linked to hull-less seed trait in pumpkin (Cucurbita pepo subsp. pepo var. styriaca)
    (Punjab Agricultural University, Ludhiana, 2016) Navjot Kaur; Dhatt, A.S.
    The investigation entitled "Genetic analysis of important economic traits in Pumpkin (Cucurbita pepo subsp. pepo var. styriaca)" was conducted to study the inheritance of important economic traits of cross namely PCK-1× Lady Godiva using generation mean analysis in the Department of Vegetable Science, Punjab Agricultural University, Ludhiana in 2014 and 2015. The experimental material comprising six generations viz. P1, P2, F1, F2, BC1P1 (F1× PCK-1) and BC1P2 (F1× Lady Godiva) was evaluated for twenty one economic traits in a randomized complete block design with three replications. Scaling tests indicated epistasis for most of the traits. The genetic parameters were estimated by applying joint scaling test and sequential model fitting after finding the best fit model. Duplicate type of epistasis was observed for days to 50% flowering, leaf length (cm), vine length (cm), inter-nodal length (cm), equatorial diameter (cm), polar diameter (cm), flesh thickness (cm), fruit yield per plant (kg), seed yield per fruit (g), number of seeds per fruit, average seed weight (g) and peduncle length (cm). Variance analysis of characters showed that additive genetic variance was predominant for leaf width, inter-nodal length, flesh thickness, number of fruits per plant, average fruit weight, average seed weight and petiole length, whereas dominance genetic variance was more in node number to first female and male flower, days to 50% flowering, number of primary branches, leaf length, vine length, days to 1st harvest, equatorial and polar diameter, fruit yield per plant, seed yield per fruit, number of seeds per fruit, fruit shape index and peduncle length. In validation of SSR molecular markers, were found linked to hull-less seed trait four markers viz. CMTm261, CMTp182, CMTm47 and CMTp257. The use of CMTm261 on BC1P1 revealed that all 75 plants were hulled, in which 32 were homozygous dominant (HH) and 37 heterozygous (Hh) for both alleles, whereas in case of BC1P2 33 plants were heterozygous (Hh) and 37 homozygous recessive (hh). The amplification of 150 F2 plants showed 29, 47 and 70 plants hulled (HH), hull-less (hh) and heterozygous (Hh) respectively. The genotypic segregation revealed that hull-less trait is controlled by single recessive gene.