Thumbnail Image

Thesis

Browse

1999 - 199912010 - 20151
Reset filters
Author: Kaur, Prabhjot × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemRestricted
    A Comparative Study of the Welfare of the Aged in Ludhiana District of Punjab State
    (Department of Economics and Sociology College of Basic Sciences and Humaniteis PAU, Ludhiana, 1999) Kaur, Prabhjot
  • Thumbnail Image
    ThesisItemOpen Access
    Biochemical basis of insecticide resistance in Spodoptera litura (Fabricius) on cole crops
    (PAU, 2015) Kaur, Prabhjot; Kang, B.K.
    Studies were conducted to monitor the magnitude of insecticide resistance in tobacco caterpillar, Spodoptera litura against different insecticides viz., emamectin benzoate, chlorantraniliprole, indoxacarb, spinosad, quinalphos and deltamethrin collected from different vegetable growing areas of Punjab. Chlorantraniliprole with LC50 value of 0.029- 0.088 ppm and emamectin benzoate with 0.037-0.081 ppm were found to be most toxic insecticides against all the tested population of S. litura followed by indoxacarb, spinosad, quinalphos and deltamethrin. Resistance levels for quinalphos and deltamethrin ranged from 21.80 to 38.90 fold and 24.8 to 64.2 fold, respectively. Different synergists i.e. Triphenyl phosphate (TPP), Diethyl maleate (DEM) and Piperonyl butoxide (PBO) at ratios of 1:1, 1:2, 1:4 and 1:6 were tested with deltamethrin to know their synergistic effect and evaluated against three different populations of S. litura collected from Malerkotla resistant (MAL), Hoshiarpur susceptible (HSP) and deltamethrin resistant Malerkotla selected strain (MAL-Sel strain) by leaf dip method. The results obtained in the present study revealed that PBO at 1:4 had highest synergistic effect on the susceptible (HSP-3.91 fold) and MAL (8.24 fold) followed by TPP and DEM (1:4). However, the stronger synergism of TPP (1:4) was 23.67 fold followed by PBO (21.30 fold) and DEM (18.26) at 1:4 respectively, in case of MAL-Sel strain which suggests the involvement of esterases and monooxygenases in imparting resistance to various insecticides. Selection with deltamethrin increased the activities of esterases and monooxygenases by 3.58 and 3.91 times in S. litura proving their role in pyrethroid mediated resisitance. Inhibition studies with quinalphos showed (2.4 times) higher tolerance in Malerkotla selected (MAL-Sel) strain than susceptible one implying the role of insensitive AchE in insecticide resistance. Activity of these enzymes and insensitivity of AchE can be used as biochemical tools for monitoring insecticide resistance in S. litura.