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20112
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Author: Jamwal, Navdeep Singh × End date: 2013 × Start date: 2011 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    MEAN AND VARIANCES AMONG F4 PROGENIES AND THEIR PREDICTION FROMPARENTAL MEANS AND GENETIC DISTANCES BASED ON MOLECULAR MARKERS IN Gossypium arboreum L.
    (Punjab Agricultural University, Ludhiana –, 2011) Jamwal, Navdeep Singh; Gumber, R.K.
    The objective of the present study was to see whether genetic distance based on SSR markers (GDSSR) and Mahalanobis D2 statistics (GDDy), parental means ( P1 + P2 /2) and absolute difference of parental means (| P1 - P2 |) can be used for predicting the means and variances of F4 populations derived from 52 crosses of Gossypium arboreum L. Fifty-two F4 populations along with 17 parents were evaluated in a randomized complete block design using three replications during Kharif 2009. Data were recorded for seed cotton yield (g), number of bolls, boll weight (g), seed index (g), halo length (mm), ginning out turn (%) and plant height (cm). Fifty-seven SSR primers belonging to BNL, MUSS, MUCS, MUSB, CIR, NAU and MGHES series detected 74 alleles in 17 arboreum lines. Total number of bands ranged from 1-3 with an average of 1.29 bands per primer. Polymorphism information content (PIC) ranged from 0.00 to 0.88 with a mean of 0.12 for all 57 primers. Maximum genetic distance based on SSR markers was observed between LD866 and AH11(0.059), whereas minimum genetic distance recorded was 0.00 between lines LD327 and MDL2643; LD694 and DLSa1001. The genetic similarity coefficient among 17 genotypes ranged from 1.00 to 0.94 with an average genetic similarity of 0.97. The maximum genetic distance (280.63) based on Mahalanobis D2 statistics was observed between LD694 and RG395, whereas minimum of 2.49 between lines LD866 and RG8. No association was found between parentage and geographical origin of parental lines with genetic distance among parental lines revealed by both the estimates of genetic divergence. Poor correlation (r=0.06) between both estimates of genetic distance GDSSR and GDDy was observed. The means of F4 populations can be predicted successfully from the means of the parents for seed cotton yield (r=0.40) and halo length (r=0.273). Likewise, | P1 - P2 | and GDSSR proved to be good predictor of F4 population means for boll weight (r=0.273) and ginning out turn (r=0.290), respectively. However, the prediction of F4 variance for most of characters except plant height (r=0.277) from different properties of parental lines still remains an unsolved problem.
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    ThesisItemOpen Access
    MEAN AND VARIANCES AMONG F4 PROGENIESAND THEIR PREDICTION FROM PARENTALMEANSAND GENETIC DISTANCES BASED ON MOLECULAR MARKERS IN Gossypium arboreum L.
    (Punjab Agricultural University, Ludhiana, 2011) Jamwal, Navdeep Singh
    The objective of the present study was to see whether genetic distance based on SSR markers (GDSSR) and Mahalanobis D2 statistics (GDDy), parental means ( P1 + P2 /2) and absolute difference of parental means (| P1 - P2 |) can be used for predicting the means and variances of F4 populations derived from 52 crosses of Gossypium arboreum L. Fifty-two F4 populations along with 17 parents were evaluated in a randomized complete block design using three replications during Kharif 2009. Data were recorded for seed cotton yield (g), number of bolls, boll weight (g), seed index (g), halo length (mm), ginning out turn (%) and plant height (cm). Fifty-seven SSR primers belonging to BNL, MUSS, MUCS, MUSB, CIR, NAU and MGHES series detected 74 alleles in 17 arboreum lines. Total number of bands ranged from 1-3 with an average of 1.29 bands per primer. Polymorphism information content (PIC) ranged from 0.00 to 0.88 with a mean of 0.12 for all 57 primers. Maximum genetic distance based on SSR markers was observed between LD866 and AH11(0.059), whereas minimum genetic distance recorded was 0.00 between lines LD327 and MDL2643; LD694 and DLSa1001. The genetic similarity coefficient among 17 genotypes ranged from 1.00 to 0.94 with an average genetic similarity of 0.97. The maximum genetic distance (280.63) based on Mahalanobis D2 statistics was observed between LD694 and RG395, whereas minimum of 2.49 between lines LD866 and RG8. No association was found between parentage and geographical origin of parental lines with genetic distance among parental lines revealed by both the estimates of genetic divergence. Poor correlation (r=0.06) between both estimates of genetic distance GDSSR and GDDy was observed. The means of F4 populations can be predicted successfully from the means of the parents for seed cotton yield (r=0.40) and halo length (r=0.273). Likewise, | P1 - P2 | and GDSSR proved to be good predictor of F4 population means for boll weight (r=0.273) and ginning out turn (r=0.290), respectively. However, the prediction of F4 variance for most of characters except plant height (r=0.277) from different properties of parental lines still remains an unsolved problem.