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Author: Davinder Kumar × Start date: 1992 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Direct shoot organogenesis and plant regeneration in Daisy and W. Murcott mandarins
    (Punjab Agricultural University, Ludhiana, 2016) Davinder Kumar; Sidhu, Gurupkar Singh
    The present investigation entitled “Direct shoot organogenesis and plant regeneration in Daisy and W. Murcott mandarins” was carried out in the Tissue Culture Laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during the year 2014-2016. Maximum seed germination of Daisy (87.2%) and W. Murcott mandarins (86.2%) on basal MS medium with minimum level of contamination (0.0%) was observed when seeds were treated with HgCl2 (0.1%) for 5 m + Bavistin (0.5%) for 10 m and HgCl2 (0.2%) treatment for 3 m respectively. In Daisy, mean maximum shoot regeneration 60.8 per cent was recorded on MS + BAP (2.0 mg/l) whereas it was maximum (74.3%) in nodes irrespective of the media used. Maximum shoot regeneration from nodal (88.4%) and root segments (62.2%) was recorded on MS fortified with BAP (1.5 mg/l). Similarly, it was maximum in internodes (40.4%) on MS + BAP (2.0 mg/l) and in leaves (78.4%) on MS + BAP (2.5 mg/l). In W. Murcott, mean maximum regeneration 74.0 per cent was recorded on MS + BAP (2.0 mg/l) irrespective of explants used and overall 81.5 per cent regeneration was recorded in nodes. Maximum shoot regeneration was recorded in nodal segments (98.2%) on MS + BAP (0.5 mg/l) + Kinetin (0.5 mg/l) and it was followed by leaf (88.8%), internodes (88.4%) and roots (36.4%) on MS + BAP (2.0 mg/l), MS + BAP (2.0 mg/l) irrespective of explant used and MS + BAP (0.5 mg/l) + Kinetin (0.5 mg/l). Maximum shoot proliferation in Daisy mandarin was observed on MS + BAP (2.5 mg/l) whereas highest number of shoots per explant was 2.60 and maximum shoot length (3.64 cm) was recorded in MS + BAP (2.5 mg/l) + GA3 (0.5 mg/l). In W. Murcott maximum proliferation was on MS + BAP (3.0 mg/l) with (2.56) shoots per explants. Whereas the maximum average shoot length of 3.46 cm was recorded on MS + BAP (2.0 mg/l) + GA3 (0.5 mg/l). Maximum rooting percentage (70.6) in Daisy mandarin were observed on ½ MS + IBA (1.0 mg/l), earliest root induction (11.2 days) and maximum number of roots per shoot (2.2) on MS + IBA (0.5 mg/l). In W. Murcott earliest root induction (12.4 days) maximum rooting (68.4%) and maximum number of roots per shoot (2.4) were observed on ½ MS + IBA (0.5 mg/l).