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Author: Baljeet Kaur × Thesis Type: M.Sc ×
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    Evaluation of germplasm and expression analysis of TFL1 gene for stem growth habit in urdbean
    (Punjab Agricultural University, Ludhiana, 2022) Baljeet Kaur; Ashok Kumar
    The present study consisted of one hundred genotypes of urdbean collected from different sources to study the stem growth habit, flowering duration and expression analysis of genes responsible for stem growth habit during different developmental stages. The experiment was conducted during spring season in 2021 at two locations (PAU, Ludhiana and PAU, RRS, Gurdaspur) in simple lattice design (10x10) using two replications. The analysis of variance showed that all the characters studied exhibit significant variation at both locations. GxE interactions were also found significant for all the characters. At Ludhiana, the characters like Plant height at first pod maturity (H2), Plant height at 90% pod maturity (H3), Number of pods per plant, Biological yield per plant, DDh1-3, DDh2_3 and DDd4_5, at Gurdaspur, Plant height at 90% pod maturity (H3), Number of pods per plant, Biological yield per plant, DDh2_3 and DD4_5 and pooled data analysis over the locations showed that Plant height at 90% pod maturity, Number of pods per plant, Biological yield per plant, yield per plant, DDh2_3 and DD4_5 possessed high PCV, GCV, heritability and GAM. The genotypic variability for all these characters are probably due to predominance of additive gene action and can be improved through phenotypic selection. The variability for these characters present in the germplasm could be exploited for developing early maturing, lodging resistant and high yielding varieties of urdbean. The expression analysis showed that TFL1 gene at three leaf stage in both the accessions i.e. IC569464 (determinate) and IC530625 (indeterminate) are similar. The expression was observed highest in flowering stage, first pod maturity and 90% pod maturity in indeterminate growth habit over determinate of plant. Specific primers were designed to check the expression of candidate gene in determinate growth habit and indeterminate growth habit at different growing stages of the plant. Hence, present study helped us to understand the molecular mechanisms of genes underlying in determinate and indeterminate growth habit of the plant in urdbean.