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Author: Avtar Singh × End date: 2014 × Start date: 2014 ×
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    ThesisItemOpen Access
    Studies on in vitro propagation of promising grape (Vitis Vinifera L.) rootstocks
    (Punjab Agricultural University, Ludhiana, 2014) Avtar Singh; Gill, M.I.S.
    The present investigation entitled “Studies on in vitro propagation of promising grape (Vitis Vinifera L.) rootstocks” was carried out in the Tissue Culture Laboratory of the Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2013-2014. The MS medium fortified with BAP (1.0 mg/l) + Kinetin (1.0 mg/l) shows maximum establishment 45.54 % and 40.80 % from nodal segments and shoot tips respectively. Rootstock R 110 and 1613 C rootstock exhibited the maximum nodal segment and shoot tip establishment 47.37 % and 35.42 % respectively. The maximum shoot regeneration (65.67 %) was recorded in MS medium supplemented with BAP (1.0 mg/l) and Kinetin (1.0 mg/l). Degrasset rootstock gave the maximum (52.89 %) shoot regeneration. Maximum number of shoots (3.63) per explant were produced at BAP (1.5 mg/l). Maximum shoot length (3.13 cm) was recorded on MS supplemented with BAP (0.5 mg/l) and Kinetin (0.5 mg/l) medium. The maximum shoot length (1.73 cm) was recorded in 1613 C rootstock. Maximum rooting was (64.95 %) recorded with MS media fortified with IBA (0.1 mg/l) and NAA (0.1 mg/l). Maximum rooting (55.64 %) was recorded in Degrasset rootstock. Maximum number of roots (13.75) was recorded in R 110 rootstock on IBA (0.01 mg/l) and NAA (0.01 mg/l). Maximum length of roots (5.66 cm) was recorded in rootstock Degrasset cultured on MS medium supplemented with IBA (0.1 mg/l). The 2:1:1 ratio of peat perlite and vermiculite and Degrasset rootstock is the best rootstock for plantlet establishment under ex vitro conditions.