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Author: Aulakh, Parjeet Singh × End date: 2019 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular mapping of nuclear male sterility gene ms10 in chilli pepper (Capsicum annuum L.)
    (Punjab Agricultural University, Ludhiana, 2016) Aulakh, Parjeet Singh; Dhaliwal, M.S.
    Most of the hybrid seed in chilli is produced manually, but use of male sterility (MS) systems can reduce cost of hybrid seed production. ‘MS-12’, a nuclear male sterile (NMS) line developed at Punjab Agricultural University, Ludhiana (India) has been utilized to develop three commercial F1 hybrids. A recessive gene designated as ms10 governs MS in ‘MS-12’. Due to recessive gene control, development of new NMS lines through the conventional backcross method is tedious and time consuming. Therefore, the present investigation was planned to identify molecular markers linked to the ms10 gene and to initiate transfer of ms10 gene into an array of elite breeding lines through marker assisted selection (MAS). Two genetically diverse parents ‘MS-12’ and ‘VR-16’ were crossed and F1 was selfed to generate F2 mapping population. A total of 558 simple sequence repeat (SSR) primer pairs were screened for parental polymorphism following bulk segregant analysis (BSA). Linkage analysis indicated that two SSR markers AVRDC- PP12 and AVRDC_MD997* were linked to the ms10 gene. Marker AVRDC-PP12 was closest to the gene at 7.2 cM distance and the marker AVRDC_MD997* was mapped at 20.8 cM from the gene. The two markers were mapped on pepper chromosome 1 at genome position 175,694,513 to 175,694,644 and 175,438,699 to 175,438,872 respectively. In backcross progenies, the marker AVRDC-PP12 distinguished the heterozygote fertile (Ms10ms10) from the homozygous dominant fertile (Ms10Ms10) plants in three segregating backcross progenies incorporated with ms10 gene. Thus, the marker AVRDC- PP12 can efficiently be used to transfer ms10 gene for marker assisted backcrossing to save time and resources. The markers provide further perspectives for fine mapping and map based cloning of the ms10 gene.