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2016 - 20192
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Author: Amanpreet Kaur × End date: 2019 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Collection, identification and domestication of wild edible Agaricales from Punjab
    (Punjab Agricultural University, Ludhiana, 2019) Amanpreet Kaur; Sodhi, H.S.
    Wild edible Agaricales were surveyed and collected during the month of July-September in the years 2015-2018 from five agro-climatic zones of Punjab. Total 127 mushrooms were collected; 34 mushrooms were collected from sub-mountain undulating zone, 19 wild mushrooms were collected from undulating plain zone, 36 mushrooms were collected from central plain zone, 17 from western plain zone, 21 from western zone. The mushrooms collected belong to genera Agaricus, Lepiota/ Macrolepiota, Pleurotus, Poadaxis, Clitocybe. Tissue culture of 92 wild mushrooms were obtained and total cellulases, cellobiase, endo-β-1,4-glucanase, endoxylanase and laccasse were estimated from pure cultures. Total cellulases ranged between 0.01 to 4.36 U/ml, cellobiase between 0.01 to 8.34 U/ml, endo-β-1,4-glucanase between 0.009 to 6.97 U/ml, endoxylanase between 0.28 to 16.18U/ml and laccasse was 0.02-3.74 U/ml. Based on enzyme producing capability, 18 cultures were identified to study linear growth (mm) on complete yeast agar medium (CYM) and potato dextrose agar (PDA) media, different pH range (5-8), temperature range (25°C-28°C). CYM was proved to be the best medium for AMN 05, AMN 71, AMN 132 and PDA was found good for AMN 14 and AMN 60. The pH 7 was proved as best pH for most of the cultures. AMN 14, AMN 39, AMN 52, AMN 54 and AMN 170 showed maximum growth at 30°C. AMN 39 and AMN 202 were successfully domesticated on wheat straw as substrate. These two cultures were subjected to molecular characterization through 18S rRNA sequencing. AMN 39 showed 100% homology with Pleurotus sapidus and AMN 202 showed the same with Pleurotus floridanus. Yield of Pleurotus sapidus (AMN 39) and Pleurotus floridanus (AMN 202) were compared with Pleurotus sajor-caju and Pleurotus florida and found that yield of Pleurotus sapidus (40 kg/q of dry substrate) and Pleurotus floridanus (47 kg/q of dry substrate) was lower than Pleurotus sajor-caju (52 kg/q of dry substrate) and Pleurotus florida (60 kg/q of dry substrate). It was observed through scanning electron microscope that hyphae of Pleurotus florida was seen to be more tightly packed as compared to Pleurotus sapidus (AMN 39) and Pleurotus floridanus (AMN 202). SDS PAGE was performed to know the heterogeneity among the mushroom proteins. AMN 39 had protein with molecular weight of 16.16 KDa and AMN 202 shown to have two proteins with molecular weight of 17.47 KDa and 42.8 KDa.
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    ThesisItemOpen Access
    Changing interaction patterns among Dalits and Jat Sikhs in Doaba region of Punjab
    (Punjab Agricultural University, Ludhiana, 2016) Amanpreet Kaur; Sharma, Shalini
    Rural population of Punjab mainly comprised of Jat Sikhs and Dalits. The land owning Jat Sikhs were locally dominant and Dalits were dependent upon them. Off late with benefits from affirmative action program of government, NRI remittances and political mobilization, Dalits had improved their socio-economic status. This had impacted the interaction patterns amongst Dalits and Jat Sikhs of the region. The present study was conducted with specific objectives: to study the socio-economic status of the respondents; to analyze the changing socio-economic and political interaction patterns among Dalits and Jat Sikhs; to identify the conflict between both castes, if any, and the reasons thereof and to investigate state intervention in conflict resolution as perceived by Dalits and Jat Sikhs. The study was based on primary data collected from 320 respondents (160 Dalits and 160 Jat Sikhs) from Doaba. Study found that almost half of Jat Sikhs (49.3%) and Dalits (43.7%) had medium socio-economic status. Three fourth of the Jat Sikhs (74.3%) and Dalits (80.0%) were from 31-50 years of age. More than one third of the respondents were educated up to higher secondary level and 42.4 per cent of Dalits were doing semi-skilled and skilled jobs whereas almost three fourth of Jat Sikhs (73.2%) were engaged in farming. It was reported that 5.6 per cent of high status Dalits had moved to the village locality inhabited by Jat Sikhs without any resistance. A significant majority of Dalits (96.2%) reported no restriction on their entry into Jat Sikh kitchen and 58.7 per cent of them attended the social ceremonies of Jat Sikhs as guests. Economic dependence on Jat Sikh patrons was reported by 19.3 per cent Dalits. In 87.5 per cent of villages there was separate Dalit gurudwaras. It was Dalit sarpanch in forty three per cent of the villages. A significant majority of Jat Sikhs were apprehensive (88.7%) of Dalit dominance while Dalits were conscious and assertive (65.6%) of their newly acquired identity. Religious issues were found most responsible behind subtle form of conflict among both castes. Two third of the respondents felt that police personnel favoured their caste group while resolving the dispute. Socially mobile, economically influential and subaltern Dalits carry the movement of a separate Dalit identity and Jat Sikhs need to understand it and act accordingly to avoid any conflict.