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Open Access
INTROGRESSION OF crtRB1 AND LcyE GENES FOR HIGH β-carotene INTO QUALITY PROTEIN MAIZE (QPM)
(Punjab Agricultural University, Ludhiana, 2018) Amandeep Kaur; Malhotra, Pawan Kumar
Maize (Zea mays L.) being the queen of cereals deficient in Vitamin A which causes the malnutrition and major health problems. Quality protein maize has enhanced level of the amino acids, lysine, and tryptophan over normal maize varieties. However, QPM varieties are low in provitamin A, a precursor of vitamin A which can lead to vitamin A deficiency in human. In the present investigation, the grain quality of QPM inbred is further enriched for β-carotene by introgressing of crtRB1 and LcyE gene through marker assisted backcross breeding. Rare natural genetic variation of crtRB1 and LcyE gene enhances β-carotene in the kernel by blocking its conversion to further components. Traditional yellow maize though contain high kernel carotenoids, but the concentration of provitamin A is quite low (<1.5µg/g) as compared to the recommended level (15µg/g). Development of biofortified maize enriched in provitamin A, lysine and tryptophan thus holds significant potential in the alleviation of micronutrients. Marker assisted stacking of crtRB1, LcyE and o2 were undertaken in the genetic background of QLM13, inbred of PMH1 hybrid. Foreground selection was carried out using gene-specific primers on BC2F1 population of QLM 13 and background selection was carried out using SSR markers to check the recovery of recurrent parent genome. The plants of favorable alleles (crtRB1 and LcyE) and 88.5-90.1% recurrent parent genome recovery were selected and selfed to generate BC2F2 population. Foreground selection was carried out on BC2F2 population using crtRB1 and LcyE gene-specific markers and plant carrying favorable homozygous allele were selfed to generate BC2F3 progenies. Quality analysis for determination of β-carotene and tryptophan analysis was carried out on BC2F3 progenies. The introgressed BC2F3 progenies possessed a high concentration of provitamin A (1.29–11.75 µg/g) as compared to recurrent parent QLM13 (4.69 µg/g). The selected lines of high beta-carotene and tryptophan content were crossed to reconstitute PMH1 hybrid. Introgressed inbred having contrast for pigmentation in glume base and silk with respective to recurrent parents possess great utility for registration and unambiguous identification in the field.
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Genetic diversity analysis using morphological and molecular markers in eggplant (Solanum melongena L.)
(Punjab Agricultural University, Ludhiana, 2018) Amandeep Kaur; Nirmaljit Kaur
Genetic diversity for different morphological traits and molecular markers of 26 eggplant genotypes including 6 hybrids was conducted at Punjab Agricultural University, Ludhiana during 2016-2018. The genotypes were characterized on the basis of 34 morphological (21 quantitative and 13 qualitative) traits and 70 SSR markers. On the basis of different quantitative traits by using Mahalanobis D2 statistic, the eggplant genotypes were grouped into six clusters with the maximum genotypes in cluster I (13 genotypes) and minimum in cluster IV and VI (one genotype in each). The maximum inter-cluster distance in quantitative traits was in cluster IV and VI (1328.581) which indicated that the genotypes in these clusters were highly diverse from each other. The minimum inter-cluster distance was observed between cluster I and II (368.376). The intra-cluster distance was maximum in cluster III (267.497) with four genotypes. The intracluster distance for the clusters IV and VI was 0.00, because of single genotype in each of these clusters. On the basis of qualitative traits, the eggplant genotypes were grouped in six clusters and the inter-cluster distances varied between15.78 to 56.43. The maximum cluster distance was in cluster IV and VI (56.431) which indicated that the genotypes in cluster IV (93SN-22-1-1-2) and VI (P-67) were highly diverse from each other. The minimum cluster distance was in cluster III and VI (15.781). The intra-cluster distance for the clusters VI and V was 0.00, because of single genotype in each of these clusters. The intra-cluster distance was maximum in cluster II (12.716) with six genotypes. Forty polymorphic markers amplified, a total of 102 alleles with an average of 2.55 alleles per locus were observed. A maximum of five alleles were amplified by emi03K06 marker. Polymorphic information content (PIC) value ranged from 0.07 to 0.78 with an average of 0.50. The highest PIC value was recorded by emi03K06 (0.78) whereas, it was minimum in emf01L14 (0.07). UPGMA based (similarity coefficient) analysis classified the eggplant genotypes into 4 clusters. Cluster I was further divided into 3 groups (IA, IB and IC). Cluster II (SC-15-2) and IV (BH-2) consisted of one genotype in each cluster. Cluster III contained two genotypes (BL-216 and BR-109). The eggplant genotypes with desirable traits can be used for successful breeding programme.
Open Access
Deciphering Morpho-physiological traits for drought tolerance of Iranian wheat (Triticum aestivum L.) landraces
(Punjab Agricultural University, Ludhiana, 2018) Amandeep Kaur; Sarlach, R.S.
The present research work on “Deciphering Morpho-physiological traits for drought tolerance of Iranian wheat (Triticum aestivum L.) landraces’’ was carried out to explore the genetic diversity of landraces for drought stress tolerance. The study was carried out at wheat laboratory and experimental area of Department of Plant Breeding and Genetics , Punjab Agricultural University, Ludhiana during two successive growing seasons of 2015-2016 and 2016-2017.To find water stress tolerance among Iranian landraces, they were subjected to different concentration of Polyethylene glycol (2%, 4%, 6%,8%,10%,14%, 16% and 18%). Among these different concentration14% PEG was found to be the suitable dose for screening, stress induced by using 14% PEG solution was best to highlight the differences in the set of germplasm.At16% and 18% most of the lines not germinated .After screening of whole set of Iranian landraces 27 landraces were selected on the basis of vigor index and evaluated under field conditions i.e. Irrigated, restricted irrigated and rain-fed conditions. The experiment was carried out in RBD design with two replications Drought environment was created by with holding of irrigation. Morpho-physiological data on plant height, tillers per meter row length, spikelet per spike, grains per spike grain yield, thousand grain weight, harvest index, chlorophyll content, stay green habit were recorded. On the basis of performance of Iranian lines under field conditions ,IWA 8600064, IWA 8600179 and PETTERSON ML68-10 considered as water stress tolerant on the basis of spikelet per spike, grains per spike thousand grain weight, grain yield and harvest index. The identified landraces can be included in future breeding programmes for the wheat improvement for drought prone areas.
Open Access
Growth and yield of pigeonpea [Cajanus cajan (L.) Millsp.] as influenced by growth retardants and sowing dates
(Punjab Agricultural University, Ludhiana, 2017) Amandeep Kaur; Harmeet Singh
The present study entitled “Growth and yield of pigeonpea [Cajanus cajan (L.) Millsp.] as influenced by growth retardants and sowing dates” was carried out at Student’s Research Farm, Department of Agronomy, Punjab Agricultural University, Ludhiana during kharif 2016. The field experiment comprising of sixteen treatments viz., control, water spray, MH @ 200, 250 and 300 ppm and TIBA @ 25, 50 and 75 ppm and two sowing dates (May 15 and May 30) was conducted in a Randomized Complete Block Design (RCBD) with three replications on sandy loam soil which was normal in pH (7.57-7.59) and electrical conductivity (0.139-0.117 dsm-1), low in organic carbon (0.23-0.26%), and available nitrogen (112.4-129.2 kg ha-1), medium in available phosphorus (18.50-21.70 kg ha-1) and available potassium (298.2-310.6 kg ha-1). May 15 sown crop produced significantly higher dry matter, LAI, number of branches, pods and grain yield than May 30 sown crop. May 15 sown produced significantly taller plants and significantly influenced CGR, RGR and LAD. Quality parameters such as N, P, K and protein content in grain and stover remained unaffected by growth retardants and sowing dates. Nodule count and weight of nodule plant-1 were not significantly affected by growth retardants and sowing dates. No effect on phenology of crop and chlorophyll content was observed by either sowing dates and growth retardant treatments. Various growth and yield parameters viz., Crop Growth Rate, Relative Growth Rate, Leaf Area Duration, dry matter, Leaf Area Index, secondary branches plant-1 and pods plant-1 were statistically higher with application of TIBA @ 75 ppm than all other growth retardant treatments and control but was statistically at par with TIBA applied @ 50 ppm. As a result, highest seed yield of pigeonpea (14.4 q ha-1) was recorded with application of TIBA @ 75 ppm although at par with foliar application of TIBA @ 50 ppm but was 22.9 per cent higher than control. Similar increase in stover yield and harvest index was also observed. No significant effect of different growth retardant sprays was found on various quality parameters like nitrogen, phosphorous, potassium and protein content of seed and stover.
Open Access
Dynamics of land use pattern and agricultural production scenario in India with particular reference to Punjab
(Punjab Agricultural University, Ludhiana, 2017) Amandeep Kaur; Kataria, Poonam
The present study, based on secondary data, was conducted to bring forth the over time changes in land use pattern of India with particular reference to Punjab. An attempt was also made to carry out the comparative evaluation of performance of agriculture and to enumerate the potential correlates of land use pattern and agricultural performance parameters in 15 major agricultural states of India for the time frame, 1980-81 to the latest available. India has geographical area of 328.7 million hectares. A close scrutiny of its land use matrix, consisting of three sub-sectors namely ecological sector, agriculture sector and non-agricultural sector, revealed that three states (Punjab, Haryana and Uttar Pradesh) have predominance of agriculture sector (more than three fourth of the reporting area). The net sown area of Punjab (2.96 % of the country’s cropped area) accounted for 97.1 per cent of the state’s agricultural land, whereas only 57.7 per cent of Tamil Nadu’s agricultural land was under plough indicating divergent patterns followed by states in the land use matrix. The cropping intensity of India increased from 123.1 per cent in 1980-81 to 138.9 per cent in 2012-13, which was far below the level attained by Punjab (189.6 %) connoting inter-state differentials in the utilization of land potential. The agricultural production performance assessed from production profile of crops crucial for food security, employment potential of agriculture, value of output and Net State Domestic Product from agriculture and allied on net area sown basis also highlighted widespread inter-state differentials. The application of Kendall’s coefficient of concordance testified that the state rankings with respect to selected land use and agricultural production parameters were in conformity (p<0.05) indicating strong association between the land use and agricultural performance parameters. The convergence analysis of agricultural performance proxied by Net State Domestic Product from agriculture and allied activities per hectare of net area sown was carried out for the four time periods:1980-81 to 1989-90, 1990-91 to 1999-00, 2000-01 to 2012-13 and overall study period. The results provided strong evidence of - convergence as indicated by estimated statistically significant coefficient for the period 2000-01 to 2012-13 implying that the states have started giving the signs of convergence post 2000 at the rate 2.7 per cent per year. The analysis has very well established that poor performing states have started catching up with the states, well known for their agricultural advancement and this would in its own right lead to food secure and prosperous India.
Open Access
Effect of elevated temperature regimes on growth and yield of wheat (Triticum aestivum) and its mitigation by agronomic management techniques
(Punjab Agricultural University, Ludhiana, 2017) Amandeep Kaur; Sidhu, Prabhjyot Kaur
A field experiment was conducted within a temperature gradient tunnel (TGT: 30m x 5m x 3m) to investigate the effect of elevated temperature regimes on growth and yield of wheat (Triticum aestivum) and its mitigation by agronomic management techniques at the Research Farm, School of Climate Change and Agricultural Meteorology, PAU, Ludhiana during rabi, 2015-16. The experiment comprised of 54 treatments combinations viz. 6 temperature regimes (+3.0, +3.2, +3.3, +3.5 and +3.6oC), 3 wheat cultivars (WH-1105, PBW-550 and HD-2967) and 3 foliar spray treatment (No foliar spray, Water spray and Urea spray 3%). The data on micrometeorological parameters, phenology, periodic growth parameters, yield and yield attributes of wheat cultivars were recorded. The foliar spray of urea 3% and foliar spray of water were applied at the late jointing and grain filling stage. The results of the study indicate that plant height and spikelet sterility increased but grain and straw yield of wheat decreased significantly with rise in temperature within the TGT. The partitioning of photosynthates among the grain and straw yield were severely affected as is indicated by the changes in harvest index. Amongst the spray treatment, the urea spray reduced the negative effect of rise in temperature more effectively as compared to water and no spray treatment. The increase in rate of spikelet sterility, plant height and decrease in rate of yield / yield attributes of wheat cultivars was mitigate effectively by the urea spray treatment. The yield and yield attributes at elevated temperature regimes was decreased maximum in cv. PBW-550 followed by cv. WH-1105 and cv. HD-2967 from ambient conditions. Amongst the wheat cultivars, HD-2967 was most tolerant and PBW-550 was most susceptible to an increase in temperature. The results of the study indicate that under anticipated increase in temperature, cv. HD-2967 may be recommended for cultivation due to its tolerant growth traits towards maintaining its yield and yield attributes under high temperature condition which are the most important determinants of high productivity of a cultivar.
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PRODUCTION OF Beauveria bassiana (BALSAMO) VUILLEMIN USING AGRICULTURAL PRODUCTS
(Punjab Agricultural University, Ludhiana, 2013) Amandeep Kaur
Beauveria bassiana isolates were mass produced on different solid substrates and effect of carrier and viability of formulation at different storage temperatures were studied. The effect of insecticides on B. bassiana growth at concentrations (one fourth MC, half MC and MC) was also studied. Seven solid substrates were evaluated for growth parameter of four Beauveria isolates and variability of the mean conidial count and colony forming unit was recorded among substrates and isolates. Among B. bassiana isolates, maximum mean conidial count (25.8×105 conidia/g) and colony forming unit (22.8×105cfu/g) was recorded in B. bassiana MTCC 6291. Among solid substrates, rice recorded maximum mean conidial count (31.8×105conidia/g) and colony forming unit (28.6×105cfu/g). Three Beauveria which were mass cultured on rice, sorghum and maize were formulated with talc, charcoal and oil as carriers. Among all the formulations maximum cfu count (44.6×105cfu/g) was recorded in talc based formulation of B. bassiana MTCC 6291 on rice. It was found that minimum per cent decrease (7.3% and 7.6% ) of viable count was recorded at 4°C in talc based formulation of B. bassiana MTCC 6291 and B. bassiana MTCC 2028, respectively. Further, to study the effect of insecticides on Beauveria growth at different concentrations, radial growth of Beauveria varied among strains. It was conclude that in all insecticides tested, radial growth varied in inverse proportion to the concentration. Lower the concentration more the radial growth recorded. Among all the insecticides tested, quinalphos was least compatible with B. bassiana strains and showed maximum mean growth percent inhibition over control (58.5%) while it was minimum in triazophos (34%).
Open Access
Title of the Thesis : Storage behaviour of plum (Prunus salicina Lindl.) fruits cv. Satluj Purple in response to various chemicals
(Punjab Agricultural University Ludhiana, 2016) Amandeep Kaur; Jawandha, S.K.
The present investigation entitled “Storage behaviour of plum (Prunus salicina Lindl.) fruits cv. Satluj Purple in response to various chemicals” was conducted in Department of Fruit Science, Punjab Agricultural University, Ludhiana during the year 2015. Plum fruits of cv. Satluj Purple were harvested at colour break stage and treated for 5-minutes in aqueous solutions of putrescine (1, 2 and 3 mmolL-1 ), boric acid (1, 2 and 3%) and sodium benzoate (0.5, 1 and 1.5%). Treated fruits were packed in corrugated fibre board (CFB) boxes with paper lining before storage at 0-1˚C temperature and 90-95% RH. Fruit samples were analysed after 7, 14, 21, 28 and 35 days of cold storage for various physico-chemical characteristics. Result revealed that physiological loss in weight (PLW) and spoilage of fruits increased with storage period, whereas fruit firmness, pulp: stone ratio and titrable acidity (TA) showed a declining trend with the advancement of storage period. Other parameters like sensory quality rating, total soluble solids, TSS: acidity ratio, total sugars, reducing sugars, non-reducing sugars and pectin methyl esterase activity (PME) showed an increase up to 21 days of storage in all the treatments except putrescine @ 3, 2 mmolL-1 and boric acid @ 3% treatments, where these parameters were increased up to 28 days of storage. Post-harvest treatments of putrescine (3, 2 mmolL-1) and boric acid (3%) proved to be effective in extending the post-harvest life of plum fruits upto 28 days, whereas sodium benzoate (1.5%) extended the post-harvest life of plum fruits upto 21 days under cold storage conditions. Postharvest treatment of putrescine @ 3 mmolL-1 was recorded most effective in reducing physiological loss in weight (PLW), spoilage, pectin methyl esterase activity (PME) and maintaining the fruit firmness, sensory quality, total soluble solids (TSS), titrable acidity (TA), total sugars, reducing sugars and non-reducing sugars during the entire storage period as compared to other treatments.
Open Access
Antioxidative defense system and grain quality evaluation of heat tolerant wheat genotypes
(Punjab Agricultural University, Ludhiana, 2014) Amandeep Kaur; Harinderjeet Kaur
The fifty genotypes of wheat in two trials Multi Location Heat Tolerance (MLHT) I and Multi Location Heat Tolerance (MLHT) II under timely and late sown conditions were sown in experimental fields of Plant Breeding and Genetics during the crop season 2011-12 to study the impact of heat stress on physiological parameters. The chlorophyll content, canopy temperature depression, days to heading, peduncle length, plant height, grain number per spike, grain weight per spike, spike number per spike, total biomass and grain yield were affected negatively under late sown conditions in all genotypes except genotypes such as GW 433, HD 3090, MP 3353, WH 1124 and RW 3705 from MLHT I and HD 3040, HI 8703, MP 3304, DBW 14, DL788-2, HUW 234 and NW 2036 from MLHT II. Genotypes GW 433, HD 3090, MP 3353 and WH 1124 from MLHT I and HD 3040, HI 8703 and MP 3304 from MLHT II were found to be less susceptible to heat stress as reflected by lower values of heat susceptibility index. Genotypes HI 1571, PBW 670, RAJ 4250 and WH 1123 from MLHT I and HI 8703, PBW 639 and WHD 946 from MLHT II were found to be susceptible to high temperature due to higher values of heat susceptibility index. Total 25 genotypes (tolerant, susceptible and checks) from both trials MLHT I and MLHT II were selected for the study of antioxidative defense system and grain quality evaluation. Genotype WH 1124 from MLHT I and genotypes HD 3040 and HI 8703 from MLHT II showed increased activity of antioxidant enzymes such as superoxide dismutase, peroxidase, ascorbate peroxidase and catalase indicated better antioxidant defense system against heat stress and hence found to be the best among the selected tolerant genotypes. GW 433, HD 3090, HD 3040 and HI 8703 from both the trials MLHT I and MLHT II showed higher grain appearance score, test weight, grain hardness, protein content, SDS-sedimentation value, grain plumpness, total sugars, dry gluten content, gluten index and better starch pasting characteristics under late sown conditions, hence can produce better quality grains. Genotypes WH 1124, HD 3090, GW 433 and HD 3040, HI 8703 from the trials MLHT I and MLHT II exhibited better antioxidative defense system and superior quality characteristics with higher yield under heat stress.

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