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20102
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Subject: Zoology × Author: Navjot Kaur × End date: 2012 × Type: Thesis ×
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    STUDY ON COMBINED EFFECT OF BROMADIOLONE AND CHOLECALCIFEROL (VITAMIN D3) AGAINST HOUSE RAT, Rattus rattus LINNAEUS
    (PAU Ludhiana, 2010) Navjot Kaur; Devinder Kaur, Kocher
    Commensal rodents, especially house rat (Rattus rattus Linnaeus) causes extensive losses by feeding and contaminating the food products and also plays a role in spreading several diseases of health importance. House rats were trapped from various commensal situations and were fed for 5 days in no-choice on standard baits of bromadiolone (0.005%) and cholecalciferol (0.075%) and their combinations having different concentrations mixed in WSO-mix bait. Male and female house rats showed 81.30% and 95.80% average acceptability of standard bait of bromadiolone over plain bait respectively. Hundred per cent mortality of male house rats was observed within 3-6 days, while all female rats died on 4th day of feeding of 0.005% bromadiolone. Both the sexes showed a significant increase in blood clotting time (sec) after 48 hours of feeding as compared to that of 0 hour. The acceptability of standard bait of cholecalciferol (0.075%) over plain bait was 40.10% and 35.30% in male and female house rats, respectively and 100% mortality was observed within 4-10 days in male and 7-14 days in female house rats. Feeding of standard bait of cholecalciferol resulted in stop feeding action in the form of significantly less consumption of this bait from 3rd day onward. Serum calcium level (mg/dL) was found to be significantly high in 0.075% cholecalciferol fed male and female rats after 48 hours of its feeding as compared to 0 hour. R. rattus when fed on four formulated baits having different concentrations of bromadiolone and cholecalciferol i.e. combination-I (0.0025% bromadiolone+0.05% cholecalciferol), combination-II (0.001% bromadiolone+0.05% cholecalciferol), combination-III (0.0025% bromadiolone+0.01% cholecalciferol) and combination-IV (0.001% bromadiolone+0.01% cholecalciferol). Out of these four tested formulated baits, combination-IV (having the lowest concentration of bromadiolone and cholecalciferol) showed efficient rodenticidal potential because of synergistic effect of bromadiolone and cholecalciferol in this combination. It was able to produce 100% mortality and showed a significant delay in blood clotting time (sec) and rise in serum calcium level and was cost effective also. R. rattus L trapped from commensal situations of Ludhiana area showed no resistance towards standard bait of bromadiolone (0.005%) as both male and female house rats (n=60) showed 100% mortality.
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    STUDY ON COMBINED EFFECT OF BROMADIOLONE AND CHOLECALCIFEROL (VITAMIN D3) AGAINST HOUSE RAT, Rattus rattus LINNAEUS
    (2010) Navjot Kaur; Kocher, Devinder Kaur
    by feeding and contaminating the food products and also plays a role in spreading several diseases of health importance. House rats were trapped from various commensal situations and were fed for 5 days in no-choice on standard baits of bromadiolone (0.005%) and cholecalciferol (0.075%) and their combinations having different concentrations mixed in WSO-mix bait. Male and female house rats showed 81.30% and 95.80% average acceptability of standard bait of bromadiolone over plain bait respectively. Hundred per cent mortality of male house rats was observed within 3-6 days, while all female rats died on 4th day of feeding of 0.005% bromadiolone. Both the sexes showed a significant increase in blood clotting time (sec) after 48 hours of feeding as compared to that of 0 hour. The acceptability of standard bait of cholecalciferol (0.075%) over plain bait was 40.10% and 35.30% in male and female house rats, respectively and 100% mortality was observed within 4-10 days in male and 7-14 days in female house rats. Feeding of standard bait of cholecalciferol resulted in stop feeding action in the form of significantly less consumption of this bait from 3rd day onward. Serum calcium level (mg/dL) was found to be significantly high in 0.075% cholecalciferol fed male and female rats after 48 hours of its feeding as compared to 0 hour. R. rattus when fed on four formulated baits having different concentrations of bromadiolone and cholecalciferol i.e. combination-I (0.0025% bromadiolone+0.05% cholecalciferol), combination-II (0.001% bromadiolone+0.05% cholecalciferol), combination-III (0.0025% bromadiolone+0.01% cholecalciferol) and combination-IV (0.001% bromadiolone+0.01% cholecalciferol). Out of these four tested formulated baits, combination-IV (having the lowest concentration of bromadiolone and cholecalciferol) showed efficient rodenticidal potential because of synergistic effect of bromadiolone and cholecalciferol in this combination. It was able to produce 100% mortality and showed a significant delay in blood clotting time (sec) and rise in serum calcium level and was cost effective also. R. rattus L trapped from commensal situations of Ludhiana area showed no resistance towards standard bait of bromadiolone (0.005%) as both male and female house rats (n=60) showed 100% mortality. Keywords: Bromadiolone, cholecalciferol, resistance, synergistic effect, R. rattus ___________________________