Loading...
Thesis
Browse
1 results
Search Results
ThesisItem Restricted Eucalyptus oil based hybrid nanoemulsions as larvicidal agents against Aedes aegypti (Linnaeus)(Punjab Agricultural University, Ludhiana, 2020) Komalpreet Kaur; Vashishat, NishaThe growing threat of vector-borne diseases and environmental pollution has prompted the nanotechnology based investigations. The present study aimed to use one of the nanotechnological applications with larvicidal potential against Aedes aegypti (Linnaeus) by preparing aqueous hybrid nanoemulsions of zinc and copper sulfide nanoparticles with non polar nanoemulsion of Eucalyptus globulosa oil by sonication. GC-MS analysis revealed presence of total 22 compounds with major composition of 1, 8-cineole (42.16%) and Pcymene (14.81%). 1:2 and 1:5 ratio of non polar oil emulsion and both nanohybrids were found to be most stable after thermodynamic stability tests. TEM studies of non polar oil nanoemulsion, CuS and ZnS based nanohybrids were found to have average size of 20-40, 80100 and 50-70 nm with spherical, globular and rectangular shapes respectively. The larvicidal activity of prepared nanoformulations were tested at different concentrations (in triplicate) and 70, 6.5 and 9.5 ppm of oil nanoemuslion, CuS and ZnS based nanohybrids were found to be the most effective concentrations as 100% mortality was observed within 24 hours. LC50 and LC90 values were calculated to be 51.95 and 64.21 ppm, 5.00 and 5.81 ppm, 7.63 and 9.22 ppm for non polar oil nanoemulsions, CuS and ZnS based nanohybrids after 24 hrs respectively. The toxicological data was assured by SEM, histological and biochemical results. Under simulated conditions, nanohybrids treatment demonstrated optimum larvicidal potency after 48 hrs of exposure. These nanohybrids did not show toxicity in Scapholebris kingi and Mesocyclops spp. Thus, the following research is a step towards safe and efficient approach against Aedes aegypti
