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20121
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Subject: Zoology × Author: KRITIKA SHARMA × End date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    HISTOPATHOLOGICAL AND BIOCHEMICAL CHANGES IN LIVER OF RATS FED Bt COTTON SEEDS
    (PUNJAB AGRICULTURAL UNIVERSITY, 2012) KRITIKA SHARMA
    The present study was undertaken to evaluate the effect of feeding Bt and non Bt cotton seeds on the liver of female albino rats. The experimental rats were divided in to three groups viz; control, Bt and non Bt group. They were fed Bt and non Bt cotton seeds at a dose of 2g/kg body weight and control rats received the same amount of water. Body weight of all the rats was taken weekly and feed intake was observed daily for four weeks. One day after the completion of experiment, the rats were sacrificed. The various vital organs i.e. liver, kidney, heart and spleen were excised and weighed. The blood was collected by cardiac puncture in heparinized vials and plasma separated for biochemical estimations. Liver was used for histopathological and biochemical estimations. It was observed that Bt cotton seeds fed rats showed more severe clinical symptoms compared to non Bt cotton seeds fed rats. The net body weight gain significantly increased in both the treated groups as compared to control rats. Average feed intake decreased significantly in non Bt cotton seeds fed rats. The weight of heart decreased significantly in both the groups while the weight of liver significantly increased in Bt cotton seeds fed rats. There was no significant difference in the weight of spleen and kidneys. Histological studies of liver of Bt cotton seeds fed rats showed leukocyte infiltration and irregular shaped hepatocytes nuclei. Non Bt cotton seeds fed rats showed swelling of hepatocytes and loose radial arrangement of hepatocytes around central vein. In the blood plasma proteins, globulin, AST, ALT and ACP increased while AKP decreased significantly in Bt cotton seeds fed rats. The protein and globulin increased significantly in non Bt cotton seeds fed rats. The level of protein, albumin and ACP, AKP, AST and ALT increased in the liver of Bt and non Bt cotton seeds fed rats. Key Words: Bt cotton seeds, non Bt cotton seeds, Albino rats, Liver, Histopathology, Enzymes, Proteins, Albumin, Globulin