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    Development of methyl anthranilate formulation to repel house rat in grain stores
    (Punjab Agricultural University, Ludhiana, 2017) Bindu Bala; Babbar, B.K.
    Testing of six different concentration of methyl anthranilate in bi-choice feeding conditions revealed that 2.5% is the effective concentration. 2.5% methyl anthranilate act both as primary (through olfaction and taste) and secondary repellent against house rats and its effect is more under bi-choice conditions. Rats do not develop habituation against 2.5% methyl anthranilate under bi-choice conditions so it can be effectively used under field conditions if alternative untreated food resources are present. Histomorphological studies of stomach, intestine and tongue revealed that 2.5% methyl anthranilate caused hyperplasia of non-glandular stomach and reduction in the number of both intestinal and gastric glands causing reduction in digestive juices and hormones secretion, degeneration of taste buds, filiform, foliate and fungiform papillae was recorded in no-choice feeding test but GIT and tongue was not affected in bi-choice feeding test. Microencapsulation of 2.5% methyl anthranilate prevented rodent attack on treated bags from 9-28 days. If treated bags were covered with tarpaulin under indoor conditions then rodent attack on bags was prevented for longer duration.