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20161
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Subject: Vegetable Science × Author: Navjot Kaur × End date: 2020 × Start date: 2000 × Type: Thesis × Thesis Type: M.Sc ×
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    Genetic analysis of important economic traits and validation of molecular markers linked to hull-less seed trait in pumpkin (Cucurbita pepo subsp. pepo var. styriaca)
    (Punjab Agricultural University, Ludhiana, 2016) Navjot Kaur; Dhatt, A.S.
    The investigation entitled "Genetic analysis of important economic traits in Pumpkin (Cucurbita pepo subsp. pepo var. styriaca)" was conducted to study the inheritance of important economic traits of cross namely PCK-1× Lady Godiva using generation mean analysis in the Department of Vegetable Science, Punjab Agricultural University, Ludhiana in 2014 and 2015. The experimental material comprising six generations viz. P1, P2, F1, F2, BC1P1 (F1× PCK-1) and BC1P2 (F1× Lady Godiva) was evaluated for twenty one economic traits in a randomized complete block design with three replications. Scaling tests indicated epistasis for most of the traits. The genetic parameters were estimated by applying joint scaling test and sequential model fitting after finding the best fit model. Duplicate type of epistasis was observed for days to 50% flowering, leaf length (cm), vine length (cm), inter-nodal length (cm), equatorial diameter (cm), polar diameter (cm), flesh thickness (cm), fruit yield per plant (kg), seed yield per fruit (g), number of seeds per fruit, average seed weight (g) and peduncle length (cm). Variance analysis of characters showed that additive genetic variance was predominant for leaf width, inter-nodal length, flesh thickness, number of fruits per plant, average fruit weight, average seed weight and petiole length, whereas dominance genetic variance was more in node number to first female and male flower, days to 50% flowering, number of primary branches, leaf length, vine length, days to 1st harvest, equatorial and polar diameter, fruit yield per plant, seed yield per fruit, number of seeds per fruit, fruit shape index and peduncle length. In validation of SSR molecular markers, were found linked to hull-less seed trait four markers viz. CMTm261, CMTp182, CMTm47 and CMTp257. The use of CMTm261 on BC1P1 revealed that all 75 plants were hulled, in which 32 were homozygous dominant (HH) and 37 heterozygous (Hh) for both alleles, whereas in case of BC1P2 33 plants were heterozygous (Hh) and 37 homozygous recessive (hh). The amplification of 150 F2 plants showed 29, 47 and 70 plants hulled (HH), hull-less (hh) and heterozygous (Hh) respectively. The genotypic segregation revealed that hull-less trait is controlled by single recessive gene.