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20112
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Subject: Plant Pathology × Author: Remander Singh × End date: 2013 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Variability and management of Alternaria brassicae (Berk.) Sacc. causing leaf blight of rapeseed and mustard.
    (Punjab Agricultural University, Ludhiana, 2011) Remander Singh
    Disease surveys revealed that disease incidence and disease severity of Alternaria leaf blight ranged from 10 to 80 per cent and 5 to 35 per cent respectively in different locations of Punjab. Based on type of symptoms, the disease samples were grouped into three categories for making isolations. In all, 25 isolates of A. brassicae were obtained. They were grouped into three groups (AB1, AB2 and AB3) based on their cultural and morphological characteristics. Representative isolates (Ab4, Ab7 and Ab14) of A. brassicae produced maximum growth and sporulation at temperature of 20±1°C on Mustard extract medium. Pathological behavior of representative isolates of A. brassicae (from each group) differed on twelve host cultivars of rapeseed and mustard. Ab5 isolate appeared to be most virulent and Ab4 isolate showed least virulence. Under in vitro conditions, Score proved most effective and completely checked the fungal growth at 10μg/ml followed by Folicur and Tilt. Indofil M-45, Antracol and Amistar were less effective. Differences in sensitivity of A. brassicae isolates were evident only at lower concentrations of Indofil M-45, Antracol and Amistar. However, they were masked at higher concentrations. Some isolates also exhibited differential sensitivity response to Score, Folicur and Tilt. In field conditions, Amistar @ 0.1 per cent proved most effective to control Alternaria leaf blight followed by Score, Folicur and Tilt each @0.1 per cent. Indofil M-45 and Antracol each @ 0.25 per cent proved less effective
  • Thumbnail Image
    ThesisItemOpen Access
    Variability and management of Alternaria brassicae (Berk.) Sacc. causing leaf blight of rapeseed and mustard.
    (Punjab Agricultural University, Ludhiana, 2011) Remander Singh
    Disease surveys revealed that disease incidence and disease severity of Alternaria leaf blight ranged from 10 to 80 per cent and 5 to 35 per cent respectively in different locations of Punjab. Based on type of symptoms, the disease samples were grouped into three categories for making isolations. In all, 25 isolates of A. brassicae were obtained. They were grouped into three groups (AB1, AB2 and AB3) based on their cultural and morphological characteristics. Representative isolates (Ab4, Ab7 and Ab14) of A. brassicae produced maximum growth and sporulation at temperature of 20±1°C on Mustard extract medium. Pathological behavior of representative isolates of A. brassicae (from each group) differed on twelve host cultivars of rapeseed and mustard. Ab5 isolate appeared to be most virulent and Ab4 isolate showed least virulence. Under in vitro conditions, Score proved most effective and completely checked the fungal growth at 10μg/ml followed by Folicur and Tilt. Indofil M-45, Antracol and Amistar were less effective. Differences in sensitivity of A. brassicae isolates were evident only at lower concentrations of Indofil M-45, Antracol and Amistar. However, they were masked at higher concentrations. Some isolates also exhibited differential sensitivity response to Score, Folicur and Tilt. In field conditions, Amistar @ 0.1 per cent proved most effective to control Alternaria leaf blight followed by Score, Folicur and Tilt each @0.1 per cent. Indofil M-45 and Antracol each @ 0.25 per cent proved less effective.