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ThesisItem Embargo Characterization of Karnal bunt (Tilletia indica) resistant genotypes and disease dynamics under alternate cultivation practices of wheat(Punjab Agricultural University, Ludhiana, 2022) Jaspreet Kaur; Vineet KumarA set of 1305 genotypes was evaluated against Tilletia indica under artificial epiphytotic conditions during cropping seasons of 2020-21 and 2021-22. After screening for two years, 284 lines were found to be highly resistant reaction and 289 lines showed resistant reaction (0-5% KB infection). Out of 284 highly resistant lines, diversity analysis of 173 Triticum aestivum lines using 70 SSR markers concluded that majority of the lines were different from each other and have potential to be explored for identification of KB resistance loci. The effect of seed and soil borne inoculum under four different tillage methods viz. happy seeder, super seeder, zero tillage and conventional (control) was studied. Super seeder (infected seed in infected soil) showed highest teliospore count (22,580 per 250 g of soil) and lowest count (1602 per 250 g of soil) was observed with zero tillage (healthy seed in healthy soil). There was significant effect of tillage methods on teliospore count. However, no significant difference of sowing dates was observed on teliospore count. The effect of seed and soil borne inoculum on germination revealed that maximum (80.66%) germination percentage was observed when healthy seed were sown in healthy soil using super seeder while minimum germination (52.26%) was observed with infected seed sown in infected soil using happy seeder. The study needs to be undertaken further to confirm the results obtained in the present investigation.
