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20211
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Subject: Plant Pathology × Author: Ashish × End date: 2021 × Start date: 2020 × Type: Thesis ×
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    Molecular mapping of resistance gene(s) for cereal cyst nematode in bread wheat (Triticum aestivum L.)
    (Punjab Agricultural University, Ludhiana, 2021) Ashish; Koulagi, Ramanna
    Wheat is one of the most important human food grains and it’s a staple food of the maximum population of the world. Heterodera avenae causing Molya disease in northern and northwestern plain zones is responsible for an annual loss of 8967.52 million rupees per year in India. To overcome this resistant breeding is the most economical and eco-friendly approach. The introgression lines were derived in background of PBW343 by crossing AUS15895. Phenotypic data was recorded for two consecutive cropping seasons (2019-20 and 2020-21). The phenotypic results revealed from both cropping seasons (2019-20 and 2020-21) that among 116 introgression lines 68 were found resistant and 10 were moderately resistant, 14 were moderately susceptible and 24 were susceptible. Upon calculation of chi square test, the BC1F6 population followed resistant 1:1 susceptible segregation ratio. Six SSR (Xwmc382, Cre5, Cre8, Cre1, CreX and Cre3) markers were employed in this study to survey the polymorphism in parents. Among the six, four markers (Xwmc382, Cre5, Cre8 and Cre1) showed the polymorphism on parents as well as on introgression lines. The marker Xwmc382 is tightly linked to resistance with highest correlation coefficient (0.1). Markers efficacy was calculated to know the efficacy rate of the markers, these results followed the same trend of marker correlation coefficient Xwmc382 is more efficient to determine the resistance against Ludhiana population of cereal cyst nematode, Heterodera avenae with 66% efficacy.