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Subject: Plant Breeding and Genetics × Author: Gaur, Amit Kumar × End date: 2019 × Start date: 2012 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Induction of genetic variability through gamma rays in pigeonpea (cajanus cajan (l.) Millspaugh)
    (Punjab Agricultural University, Ludhiana, 2016) Gaur, Amit Kumar; Inderjit Singh
    The present investigation was conducted to induce genetic variability in yield contributing traits in local variety AL 201 and to isolate desirable mutants for important traits which can be used as such as new varieties or as parents in hybridization programme for developing superior varieties. Two doses of gamma radiation i.e. 30 kR and 40 kR were used to induce mutations and the experiments were conducted in the experimental farm area of Pulses Section, Deptt. of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, during kharif season of 2014 and 2015. The effect of gamma rays treatment on the per cent seed germination was studied in M1 generation under both laboratory as well as field conditions and results indicated that the gamma rays had an inhibitory effect on the per cent seed germination of pigeonpea and these negative effects was more severe with increasing dose of the mutagen. In M2 generation, mutant plants were selected and were divided into different groups for desirable traits like earliness, dwarfness, profuse branching and more yield per plant for both the doses i.e 30 kR and 40 kR. For these traits mean of selected plants was significantly different from mean of control plants. Among all the groups, group V (30kR) was considered as the best as it contained early maturing plants, dwarf plants, plants with more fruiting branches and high yield than the parent AL 201. A set of 20 already identified M3 derived M4 mutant lines were evaluated for various morphological traits and protein content. Analysis of variance revealed that mean squares were significant for all the traits. Mutant genotype AL 2138 was identified as the shortest and earliest line having more yield than the parent variety AL 201. The other mutant lines AL 2127, AL 2128, were found promising for pods per plant, 100-seed weight, fruiting branches per plant and seed yield. The highest value of phenotypic coefficient of variation (PCV) was obtained for seed yield per plot whereas, the highest genotypic coefficient of variation (GCV) was recorded for fruiting branches. High heritability estimates were reported for days to maturity and days to flowering. The highest genetic advance as per cent of mean was recorded for number of fruiting branches per plant. Seed yield per plot recorded positive and significantly high correlation with fruiting branches, pods per plants, 100-seed weight, plant height, days to flowering and days to maturity.