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Subject: Plant Breeding × Start date: 2020 × Type: Thesis ×
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    ThesisItemOpen Access
    Inheritance and molecular mapping of cotton leaf curl disease resistance in synthetic amphiploid × Gossypium hirsutum cross
    (Punjab Agricultural University, Ludhiana-, 2020) Vij, Suruchi; Pathak, Dharminder
    The present investigation was undertaken with the objectives to study inheritance of cotton leaf curl disease and to map the genes imparting CLCuD resistance. For this, the available CLCuD resistant F1 hybrids between a 'Synthetic polyploid' and G. hirsutum Acc. PIL 43 were used for the development of BC1F1 population using PIL 43 as the recurrent parent. CLCuD resistance was dominant over its susceptibility. Two genes were found to govern resistance to CLCuD as revealed by the appearance of resistant and susceptible plants in the ratio of 1:3 in the BC1F1 generation. The CLCuD resistance genes were mapped on chromosome A01 and D07. SNP marker SD07_2729958 was found to be associated with the target gene at a distance of 24.8 cM on chromosome D07. The gene on chromosome A01 was flanked by markers SA01_115554458 and SA01_288632 which were 41.1 cM and 37.3 cM away from the target gene, respectively. The linkage map spanned a length of 12606.89 cM with an average distance of 6.82 cM between adjacent markers. Similar recombination rates were observed in the A- and D-subgenomes. A set of 210 BC1F3 progenies were evaluated along with four checks (PIL 43, LH 2108, F 2228 and F 2164) for yield and fibre traits. Nineteen progenies having tenacity in the range of 31.3 g/tex through 34.7 g/tex were observed to be significantly superior to the recurrent parent (28.8 g/tex). Similarly, progenies outperforming the recurrent parent for fibre length and fineness were also recovered.