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ThesisItem Open Access Inheritance and molecular mapping of cotton leaf curl disease resistance in synthetic amphiploid × Gossypium hirsutum cross(Punjab Agricultural University, Ludhiana-, 2020) Vij, Suruchi; Pathak, DharminderThe present investigation was undertaken with the objectives to study inheritance of cotton leaf curl disease and to map the genes imparting CLCuD resistance. For this, the available CLCuD resistant F1 hybrids between a 'Synthetic polyploid' and G. hirsutum Acc. PIL 43 were used for the development of BC1F1 population using PIL 43 as the recurrent parent. CLCuD resistance was dominant over its susceptibility. Two genes were found to govern resistance to CLCuD as revealed by the appearance of resistant and susceptible plants in the ratio of 1:3 in the BC1F1 generation. The CLCuD resistance genes were mapped on chromosome A01 and D07. SNP marker SD07_2729958 was found to be associated with the target gene at a distance of 24.8 cM on chromosome D07. The gene on chromosome A01 was flanked by markers SA01_115554458 and SA01_288632 which were 41.1 cM and 37.3 cM away from the target gene, respectively. The linkage map spanned a length of 12606.89 cM with an average distance of 6.82 cM between adjacent markers. Similar recombination rates were observed in the A- and D-subgenomes. A set of 210 BC1F3 progenies were evaluated along with four checks (PIL 43, LH 2108, F 2228 and F 2164) for yield and fibre traits. Nineteen progenies having tenacity in the range of 31.3 g/tex through 34.7 g/tex were observed to be significantly superior to the recurrent parent (28.8 g/tex). Similarly, progenies outperforming the recurrent parent for fibre length and fineness were also recovered.ThesisItem Open Access Assessment of Genetic Potential of New Inbred lines of Maize (zea Mays L.) as Partners of Different heterotic Pools(Department of Plant Breeding PAU, Ludhiana, 2000) Sandhu, Shivtej SinghThesisItem Open Access Genetic analysis of fodder traits in pearl millet (pennisetum glaucum (L.) R. Br(Department of Plant Breeding PAU, Ludhiana, 2002) Singh, Satinder; Bhardwaj, B. LThesisItem Open Access Hybridization between crop brassicas and some relted crucifers(Department of Plant Breeding PAU, Ludhiana, 2001) Bhaskar, P. Bala; Banga, S.SThesisItem Open Access ESTIMATION OF GENETIC DIVERSITY USING SSRs IN MAIZE INBRED LINES IN RELATION TO HETEROSIS AND COMBINING ABILITY(PAU Ludhiana, 2013) Nivgojind Singh Virk; S P S, BrarThe present investigation was conducted to estimate the magnitude of general and specific combining ability of inbred lines and crosses respectively and heterosis for yield and yield component characters of maize. Forty four F1 crosses were made in a line x tester fashion by using twenty two lines and two testers and all crosses with their parents were evaluated. The data was recorded on nine characters. The lines 3(LM13), 26-2009 and 37-2009 were good general combiners for most of the characters studied. The estimates of gca effects revealed that lines 10-2009, 22(LTP-4), 24-2009, 38-2009, 39-2009, 41(CML32), 95-2009 were good general combiners for grain yield/plant. The hybrids 29-2009xLM13, 37-2009xLM13, 97-2009xLM13, 9-2009xLM14, 97-2009xLM14 were found to be good specific combiners for most of the characters. Fourteen hybrids were found to be good specific combiners for grain yield/plant. The hybrids 3(LM11)xLM13, 3(LM11)xLM14, 13-2009xLM14, 22(LTP-4)xLM14, 24-2009xLM13, 24-2009xLM14, 37-2009xLM14, 39-2009xLM13, 41(CML32)xLM14, 45-2009xLM14, 46-2009xLM14, 59-2009xLM14, 95-2009xLM13, 95-2009xLM14 were found to be good cross combinations which showed significant mid parent heterosis for most of the characters. Unweighted pair group method with arithmetical averages (UPGMA) available in numerical taxonomic and multivariate analysis system (NTSYS-pc) 2.02e was used to group the inbred lines in different clusters. Dendrogram constructed grouped the inbred lines in two clusters (C-1, C-2). But the morphological grouping of parents was not in agreement with the heterotic pattern of their crosses. A set of twelve SSR primers dispersed throughout the maize genome was used to assess the genetic diversity at the molecular level. A total of forty alleles were detected and polymorphism information content (PIC) value ranged from 0.26 to 0.81 with an average value of 0.5. SSR primers with di-repeats revealed a maximum mean PIC value of 0.56, followed by tetrarepeats (0.44) and tri-repeats (0.39). It was able to assign inbred lines to three clusters (C-1, C-2 and C-3). In order to predict the hybrid performance, utilizing SSR markers, genetic distances were calculated among the inbred lines. Correlation coefficients calculated between the genetic distances of inbred lines and mid parent heterosis and specific combining ability for various yield and yield component characters of maize were non significant. Thus it seems rather difficult to make a generalized conclusion regarding the prediction of hybrid performance especially for grain yield, based on genetic distance measures.ThesisItem Open Access HETEROSIS, COMBINING ABILITY AND CORRELATION STUDIES IN THREE-WAY CROSS HYBRIDS IN SUNFLOWER (Helianthus annuus L.)(Punjab Agricultural University, Ludhiana, 2013) Yadvinder SinghStudy was undertaken to assess the magnitude of heterosis and combining ability in three-way cross hybrids of sunflower. A set of eleven A lines 302A X 11B, 302A X 42B, 302A X 234B, 302A X 44B, 44A X 40B, 11A X 234B, 43A X 44B, 234A X 40B, 44A X 234B, 44A X 42B, 44A X 11B and four restorers P89R, P69R, P93R and P110R were crossed in all possible combinations to obtain 44 TWC hybrids to evaluate for general combining ability (GCA) and specific combining ability (SCA) in a randomized block design over two years in spring 2011 and spring 2012. Genetic variability among genotypes was assessed for days to flower initiation, days to 50% flower initiation, days to maturity, plant height, head diameter, no. of leaves per plant, chlorophyll content, leaf area, leaf area index, seed yield per plant, 100 seed weight, oil content, stay green and fatty acid composition. CMS 11A X 234B had significant general combining ability effects for seed yield, head diameter, chlorophyll content, leaf area and oil content followed by CMS 44A X 40B for seed yield, plant height and days to flower initiation. Among the restorers P89R was good combiner for seed yield, head diameter, no. of leaves, chlorophyll content and 100 seed weight followed by P93R for head diameter, leaf area index, 100 seed weight and oil content. Two crosses 302A X 44B X P89R and 302A X 234B X P69R had significant sca effects for various characters. Importance of additive gene effects was found in the inheritance of traits like plant height and 100 seed weight. However for all other characters such as days to flower initiation, days to maturity, head diameter, no. of leaves, chlorophyll content, leaf area, leaf area index, seed yield and oil content non-additive gene effects predominated. Hybrids 302A X 44B X P89R, 11A X 234B X P89R and 234A X 302B X P93R excelled the standard checks for most of the important traits. The hybrids in general involving the parents 234A X 44B, 234A X 302B, P93R and P89R exhibited high positive heterosis for seed yield per plant. High significant correlation was observed between seed yield and 100 seed weight and plant height followed by head diameter, no. of leaves, chlorophyll content, leaf area and leaf area index. Keywords: Heterosis, combining ability and three way cross hybrid.ThesisItem Restricted Microspore Culture And Agrobacterium Mediated Genetic Transformation In Rice(Punjab Agricultural University ;Ludhiana, 2001) Acharya, Santanu; Chahal, G. S.ThesisItem Open Access Exploitation Of Wild Species For Genetic Improvement Of Chickpea(Punjab Agricultural University ;Ludhiana, 2001) Bains, Tejinderjit Singh; Sidhu, P. S.ThesisItem Open Access Interspecific Hybridization And Induced Mutagenesis To Generate Variability For Key Economic Traits In Brassica Carinata A. Braun(Punjab Agricultural University; Ludhiana, 2006) Sheikh, Farooq Ahmad; Banga, Shashi
