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Subject: Plant Breeding × Start date: 1966 × Thesis Type: Ph.D ×
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Now showing 1 - 9 of 20
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    ThesisItemOpen Access
    Inheritance and molecular mapping of cotton leaf curl disease resistance in synthetic amphiploid × Gossypium hirsutum cross
    (Punjab Agricultural University, Ludhiana-, 2020) Vij, Suruchi; Pathak, Dharminder
    The present investigation was undertaken with the objectives to study inheritance of cotton leaf curl disease and to map the genes imparting CLCuD resistance. For this, the available CLCuD resistant F1 hybrids between a 'Synthetic polyploid' and G. hirsutum Acc. PIL 43 were used for the development of BC1F1 population using PIL 43 as the recurrent parent. CLCuD resistance was dominant over its susceptibility. Two genes were found to govern resistance to CLCuD as revealed by the appearance of resistant and susceptible plants in the ratio of 1:3 in the BC1F1 generation. The CLCuD resistance genes were mapped on chromosome A01 and D07. SNP marker SD07_2729958 was found to be associated with the target gene at a distance of 24.8 cM on chromosome D07. The gene on chromosome A01 was flanked by markers SA01_115554458 and SA01_288632 which were 41.1 cM and 37.3 cM away from the target gene, respectively. The linkage map spanned a length of 12606.89 cM with an average distance of 6.82 cM between adjacent markers. Similar recombination rates were observed in the A- and D-subgenomes. A set of 210 BC1F3 progenies were evaluated along with four checks (PIL 43, LH 2108, F 2228 and F 2164) for yield and fibre traits. Nineteen progenies having tenacity in the range of 31.3 g/tex through 34.7 g/tex were observed to be significantly superior to the recurrent parent (28.8 g/tex). Similarly, progenies outperforming the recurrent parent for fibre length and fineness were also recovered.
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    ThesisItemOpen Access
    Combining ability and genetic parameters in mungbean (Phaseolus areus roxb.)
    (Punjab Agricultural University, Ludhiana, 1970) Singh, Tejinder Pal; Singh, K. B
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    Microspore Culture And Agrobacterium Mediated Genetic Transformation In Rice
    (Punjab Agricultural University ;Ludhiana, 2001) Acharya, Santanu; Chahal, G. S.
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    ThesisItemOpen Access
    Exploitation Of Wild Species For Genetic Improvement Of Chickpea
    (Punjab Agricultural University ;Ludhiana, 2001) Bains, Tejinderjit Singh; Sidhu, P. S.
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    ThesisItemOpen Access
    Interspecific Hybridization And Induced Mutagenesis To Generate Variability For Key Economic Traits In Brassica Carinata A. Braun
    (Punjab Agricultural University; Ludhiana, 2006) Sheikh, Farooq Ahmad; Banga, Shashi
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    ThesisItemOpen Access
    Combining Ability Estimates And Study Of Genetic Diversity Among Maize (Zea Mays L.) Inbred Lines Using Microsatellite Markers
    (Punjab Agricultural University; Ludhiana, 2006) Bhat, Mohd. Ashraf; Malhi, N.S.
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    Studies on somatic embryogenesis and genetic transformation in maize
    (PAU, 2012) Dhillon, Navjot Kaur; Gosal, S.S
    The present investigation entitled, “Studieson somatic embryogenesis and genetic transformation in maize” was undertaken using maize inbreds viz., LM 5, LM 6, LM 13, LM 15 and LM 16. Callus induction was studied on ten media compositions based on MS (1962) salts using three explants, viz., immature embryos, mature embryos and split seeds. Among five maize inbreds thus investigated, LM 13 was most responsive to tissue culture. Highest (88.7%) callusing was observed from immature embryos cultured on MS + 2,4-D (3.0 mgL -1 ) + Picloram (10.0 mgL -1). Whereas, the maximum (88.6%) callus induction using mature embryos was observed on MS + Picloram (10.0 mgL -1 ) + BAP (0.5 mgL -1 ). Subcultured calli exhibited frequent somatic embryogenesis and the highest (72.6%) somatic embryogenesis was observed in inbred LM 13 on MS + Picloram (10.0 mgL -1 ) + BAP (0.3 mgL -1 ). The addition of proline, casein hydrolysate, silver nitrate and sucrose enhanced somatic embryogenesis in all the calli. Whereas, the cefotaxime did not have much effect on somatic embryogenesis. Embrogenic calli, upon their transfer to regeneration medium, exhibited shoot bud/shoot and root regeneration. The maximum (71.6%) shoot regeneration in LM 13 from immature embryo-derived calli was achieved on MS + Proline (3.0 mgL -1 ) + BAP (3.0 mgL -1 ) + Kin (0.5 mgL -1 ) + Gelrite (2.0%). Whereas, the mature embryo-derived calli of same inbred cultured on same regeneration medium exhibited poor (26.6%) shoot regeneration. Genetic transformation was attempted with particle gun (Bio Rad) using two target tissues viz. embroygenic calli and immature embryos. The bombardment was done using plasmids carrying glyI, glyII genes (for abiotic stress tolerance) and Cry1A(c) gene (for insect resistance). The bombarded tissues were selected using hygromycin (35 ppm) for two cycles of selection of two weeks each. In case of glyIIgene bombardment, a total 146 plants were regenerated from which 42 plants (43.29%) were GUS positive. Further, the PCR analysis revealed the presence of glyII gene in 6 plants (4.10%). Whereas, in case of Cry1A(c) gene, a total 171 immature embryos were bombarded from which 2 plants (1.16%) were found to bePCR positive. The tissue cultured regenerated plants from all the bombarded tissues were grown in the transgenic glass house for collection of seeds. None of the plants carrying glyII gene set any seed. Whereas, four seeds have been collected from a plant carryingCry1A(c) gene.
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Expression, Inheritance And Molecular Tagging Of Male Fertility Restorer Gene(S) In Brassica Napus L.
    (Punjab Agricultural University ;Ludhiana, 2001) Harmeet Singh; Banga, S. S.