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Subject: Plant Breeding × Author: Choudhary, Khushwant B. × Start date: 2012 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    MORPHO-AGRONOMIC AND MOLECULAR CHARACTERIZATION OF GENETIC DIVERSITY IN OAT (Avena sativa L.) GERMPLASM
    (Punjab Agricultural University, Ludhiana, 2013) Choudhary, Khushwant B.; Kapoor, Rahul
    The present study was undertaken to assess the genetic diversity present in the ninety six oat (Avena sativa L.) germplasm lines representing the collection from various eco-geographical regions of the country. On the basis of mean performance the lines viz; OL 1684 for plant height (121.80 cm), SKO 109 and OL 1693 for leaf length (69.65 cm) and leaf width (3.30 cm) respectively, OL 1615 and OL 1612 for GFY (89.50 and 88.00 kg/plot) and DMY (32.20 and 31.75 kg/plot) were found to be superior. Mahalanobis D2 analysis grouped all 96 genotypes into ten clusters. Cluster VII (26 genotypes) and Cluster IX (1 genotypes) were the largest and the smallest clusters respectively amongst all. The mean for GFY (84.50 kg/plot), DMY (29.84 kg/plot) was highest in cluster number V, whereas cluster VI had highest mean for leaf length (58.89 cm), leaf width (2.29 cm), days to 50% flowering (129.75) and days to maturity (159.75). The inter-cluster distance was highest between VI and IX cluster pair (25.87) followed by VI and X (21.51) suggesting significant high genetic diversity among genotypes of these clusters. The molecular diversity analysis using 40 SSR markers clustered all the 96 germplasm lines into ten clusters and significant level of dissimilarity (0.12 to 0.96) was depicted among the lines. Genotypic pairs having utmost genetic dissimilarity (0.96) were OL 1634 and OL1688, OL1702 and OL1688, OL 1705 and OL 1634, UPO 03-3 and OL 1688, UPO 03-3 and OL1705. PIC values ranged from as low as 0.06 to as high as 0.75 (AM 7). The primer pairs with highest PIC value were AM 7(0.75), AM2 (0.69) and AM10 (0.69). In addition, statistically non significant correlation with r=0.195 (P=0.05) was reported between morpho- agronomic and molecular diversity.