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Subject: Microbiology × Author: Ramandeep Kaur × End date: 2020 ×
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    Effect of growth regulators on biomass production and yield potential of milky mushroom Calocybe indica (P & C)
    (Punjab Agricultural University, Ludhiana, 2016) Ramandeep Kaur; Sodhi, Harpreet Singh
    Calocybe indica also known as ‘milky mushroom’ is an edible tropical mushroom originated from India. It is cultivated indoor on wheat/paddy straw during the summer season (30±2°C). This study was aimed to increase the biomass production and yield potential of Calocybe indica using three growth regulators namely Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and gibberellic acid (GA). Out of three media namely Complete Yeast extract Medium (CYM), Potato Dextrose Agar medium (PDA) and Wheat Extract Agar medium (WEA), the maximum colony diameter (mm) and growth rate (mm/day) was observed on WEA medium supplemented with growth regulators (IAA @ 10 ppm, IBA @ 10, 25 ppm and GA @ 50 ppm) followed by CYM. The biomass production was maximum in broth supplemented with GA for all the media with maximum (24.2 g/l) in CYM. Maximum yield was obtained with GA when sprayed at pin head formation (64.3 kg/q) and at all stages (71.1 kg/q); with IBA at 1st flush (75.9 kg/q) and at all stages (70.9 kg/q); with IAA at 1st flush (58.2 kg/q) and at all stages (62.1 kg/q). Supplementation of growth regulators in substrate at the time of spawning showed no significant variation in the yield potential of milky mushroom. The treatment of growth regulators showed no significant effect on the quality parameters. The mushrooms treated with growth regulators like the untreated ones can be stored for 2-3 days at ambient temperature and upto 14 days in refrigerator in polypropylene and polyethylene bags. An increase in the nutritional components of fruit bodies sprayed with IAA and GA was also observed. The dried mushroom slices could be stored upto 3 months in polyethylene bags without any significant change in colour, texture, nutrition and bacterial count. Among the three drying temperatures (45°, 55° and 65°C) 55°C was found best as it resulted in good colour and texture of fruit bodies with no loss in nutritional components. The in vitro protein digestibility of spent mushroom substrate was found in the range of 53.34-58.21 % on 100 gram dry weight basis. Thus, it can also be used as animal feed as it contained high protein content.