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NUTRITIONAL,BIOCHEMICAL AND CULTIVATION STUDIES OF REISHI MUSHROOM (Ganoderma lucidum)
(Punjab Agricultural University, Ludhiana, 2007) Kaur, Manpreet; Khanna, P.K.
The conditions for biomass production of two strains of Ganoderma lucidum GLS and GLM in liquid medium were optimized. Mushroom complete medium, 25°C temperature, 12 hr light exposure/day and supplementation with malt extract and ammonium chloride were the four optimized parameters for maximum biomass production. Proximate chemical composition of mycelium of two strains was determined and it contained 27.5-30.2 per cent carbohydrates, 10.5-12.2 per cent protein, 5.5-6.0 percent fat, 4.0-4.9 per cent ash and 46.7-52.5 per cent fibres. The strains were evaluated for production of lignocellulolytic enzymes. Maximum Endo β-D 1,4 glucanase (CMCase) and xylanase activity was observed in GLS strain whereas maximum β-glucosidase (Cellobiase) activity was observed in GLM strain. Growth behaviour of mycelium on solid substrate i.e. wheat straw and saw dust was studied. Maximum spawn run was observed on 20% wheat bran supplemented wheat straw with 1.75 mm/day mycelial extension rate in GLS strain. It took 49 days for impregnation for substrate with mycelium of G. lucidum.