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Subject: Microbiology × Author: Kaur, Jatminder × End date: 2013 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Submitted to the Punjab Agricultural University in partial fulfilment of the requirements for the degree of
    (Punjab Agricultural University, Ludhiana, 2013) Kaur, Jatminder; Gupta Phutela, Urmila
    Ethanol is the most consumed biofuel in the world. Sweet sorghum [Sorghum bicolor (L.) Moench], a C4 Graminaceous crop which has sugar rich stalks having good potential as an alternative feed stock for ethanol production. In present study the sweet sorghum juice of variety CSV19SS was procured and was being analyzed for storage stability and its fermentation potential. Sweet sorghum juice was found to be stable under refrigeration conditions for about 75 days without any significant changes in the sugar profile and acidity of the juice. Two Saccharomyces cerevisiae strains namely S. cerevisiae strain-35 and S. cerevisiae strain NRRL Y-2034 were used for fermentation of sweet sorghum juice. S. cerevisiae strain-35 was found to be more efficient yielding 8.6% ethanol with fermentation efficiency of 86.69% as compared to S. cerevisiae strain NRRL Y-2034 yielding 8.4% ethanol with fermentation efficiency of 86.34%. Optimization of cultural parameters for maximum ethanol production and fermentation efficiency was done with the help of statistical software Statgraphics Centurian XVI.I using Response Surface Methodology. Various optimizing parameters were inoculum concentration, agitation rate and temperature and the resultant responses were ethanol content, total acidity and pH, using sugarcane and sweet sorghum juice inoculated separately by Saccharomyces cerevisiae strain NRRL Y-2034 and Saccharomyces cerevisiae strain-35. Production of ethanol from sweet sorghum juice and sugarcane juice (COJ-89) was compared under optimized conditions. Sweet sorghum juice inoculated with S. cerevisiae NRRL Y-2034 yielded 8.85% (v/v) ethanol with fermentation efficiency of 87.35% under optimized conditions of temperature (30ºC), agitation rate (50 rpm) and inoculum size (7.5% v/v). The sugarcane juice inoculated with S. cerevisiae NRRL Y-2034 yielded 10.14% (v/v) ethanol under optimized conditions of temperature (30ºC), agitation rate (100 rpm) and inoculum size (5% v/v) with fermentation efficiency of 97.67%. When sweet sorghum juice inoculated with S. cerevisiae Strain-35 8.47% (v/v) ethanol was produced under optimized conditions of temperature (30ºC), agitation rate (50 rpm) and inoculum size (5% v/v) with fermentation efficiency of 83.56%. However in sugarcane higher ethanol production was observed (11.23% v/v) with fermentation efficiency of 95.62% under optimized conditions of temperature (30ºC), agitation rate (100 rpm) and inoculum size (7.5% v/v). Sweet sorghum juice and sugarcane juices are good substrates for ethanol production. However, lifecycle assessment of both the crops has an edge towards the utilization of sweet sorghum juice for ethanol production
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    ThesisItemOpen Access
    PROCESS OPTIMIZATION FOR ETHANOL PRODUCTION FROM SWEET SORGHUM JUICE USING Saccharomyces cerevisiae
    (Punjab Agricultural University, Ludhiana, 2013) Kaur, Jatminder; Gupta Phutela, Urmila
    Ethanol is the most consumed biofuel in the world. Sweet sorghum [Sorghum bicolor (L.) Moench], a C4 Graminaceous crop which has sugar rich stalks having good potential as an alternative feed stock for ethanol production. In present study the sweet sorghum juice of variety CSV19SS was procured and was being analyzed for storage stability and its fermentation potential. Sweet sorghum juice was found to be stable under refrigeration conditions for about 75 days without any significant changes in the sugar profile and acidity of the juice. Two Saccharomyces cerevisiae strains namely S. cerevisiae strain-35 and S. cerevisiae strain NRRL Y-2034 were used for fermentation of sweet sorghum juice. S. cerevisiae strain-35 was found to be more efficient yielding 8.6% ethanol with fermentation efficiency of 86.69% as compared to S. cerevisiae strain NRRL Y-2034 yielding 8.4% ethanol with fermentation efficiency of 86.34%. Optimization of cultural parameters for maximum ethanol production and fermentation efficiency was done with the help of statistical software Statgraphics Centurian XVI.I using Response Surface Methodology. Various optimizing parameters were inoculum concentration, agitation rate and temperature and the resultant responses were ethanol content, total acidity and pH, using sugarcane and sweet sorghum juice inoculated separately by Saccharomyces cerevisiae strain NRRL Y-2034 and Saccharomyces cerevisiae strain-35. Production of ethanol from sweet sorghum juice and sugarcane juice (COJ-89) was compared under optimized conditions. Sweet sorghum juice inoculated with S. cerevisiae NRRL Y-2034 yielded 8.85% (v/v) ethanol with fermentation efficiency of 87.35% under optimized conditions of temperature (30ºC), agitation rate (50 rpm) and inoculum size (7.5% v/v). The sugarcane juice inoculated with S. cerevisiae NRRL Y-2034 yielded 10.14% (v/v) ethanol under optimized conditions of temperature (30ºC), agitation rate (100 rpm) and inoculum size (5% v/v) with fermentation efficiency of 97.67%. When sweet sorghum juice inoculated with S. cerevisiae Strain-35 8.47% (v/v) ethanol was produced under optimized conditions of temperature (30ºC), agitation rate (50 rpm) and inoculum size (5% v/v) with fermentation efficiency of 83.56%. However in sugarcane higher ethanol production was observed (11.23% v/v) with fermentation efficiency of 95.62% under optimized conditions of temperature (30ºC), agitation rate (100 rpm) and inoculum size (7.5% v/v). Sweet sorghum juice and sugarcane juices are good substrates for ethanol production. However, lifecycle assessment of both the crops has an edge towards the utilization of sweet sorghum juice for ethanol production.