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Subject: Microbiology × Author: Harpreet Kaur × Start date: 1998 × Thesis Type: M.Sc ×
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    Pretreatment of paddy straw by Delftia sp. for enhancing biogas production
    (Punjab Agricultural University, Ludhiana, 2018) Harpreet Kaur; Phutela, Urmila Gupta
    The present study was aimed to study the growth profile and lignocellulolytic enzyme (laccase, endoglucanase, exoglucanase and -glucosidase) profile of Delftia sp. PP4_S3 (bacteria) which was used for pretreatment of paddy straw. The pretreated paddy straw was used for enhancing biogas production. The comparison of biogas production from bacterial (Delftia sp. PP4_S3) and fungal (Coriolus versicolor MTCC 138) pretreated paddy straw also carried out. Maximum growth of Delftia sp. PP4_S3 was obtained after 72 h with OD (600nm) value of 1.890±0.27 and cell count of 8.76×107±0.02 in paddy straw based nutrient broth. In solid state fermentation, maximum laccase, endoglucanase, exoglucanase and - glucosidase activities of 203 Ug-1, 35.7 Ug-1 , 14.2 Ug-1 and 11.3 Ug-1 respectively were obtained as compared to 11.2 Uml-1, 6.46 U/ml-1, 1.85 U/ml-1 and 2.13 U/ml in submerged conditions. The pretreatment of paddy straw with Delftia sp. PP4_S3 showed maximum reduction of 7.46% total solids, 5.67% volatile solids, 40.6%cellulose, 22.5% hemicellulose, 45.7% lignin and 17.7% silica in 5 days of pretreatment period. Highest biogas production i.e. 180 l/kg PS, 560 l/kg TS and 673.2 l/kg VS (66.1% enhanced over control) was found on 3rd day of treatment. Paddy straw pretreated with Coriolus versicolor MTCC 138 showed 44.7% increased biogas production with 153.4 l/kg PS, 561.9 l/kg TS and 689.9 l/kg VS. Pretreatment of paddy straw with Coriolus versicolor MTCC 138 showed maximum reduction of 3.71% total solids, 4.61% volatile solids, 38.6%cellulose, 14.8% hemicellulose, 42.6% lignin and 16.2% silica. Both Delftia sp. PP4_S3 and Coriolus versicolor MTCC 138 showed significant reduction in lignin and silica content which indicate that both are lignin and silica degrader. However less pretreatment period required by bacteria make it more suitable for biogas production.