Loading...
![Thumbnail Image](assets/images/Collection.png)
Thesis
Browse
3 results
Search Results
ThesisItem Restricted Pretreatment of paddy straw by Delftia sp. for enhancing biogas production(Punjab Agricultural University, Ludhiana, 2018) Harpreet Kaur; Phutela, Urmila GuptaThe present study was aimed to study the growth profile and lignocellulolytic enzyme (laccase, endoglucanase, exoglucanase and -glucosidase) profile of Delftia sp. PP4_S3 (bacteria) which was used for pretreatment of paddy straw. The pretreated paddy straw was used for enhancing biogas production. The comparison of biogas production from bacterial (Delftia sp. PP4_S3) and fungal (Coriolus versicolor MTCC 138) pretreated paddy straw also carried out. Maximum growth of Delftia sp. PP4_S3 was obtained after 72 h with OD (600nm) value of 1.890±0.27 and cell count of 8.76×107±0.02 in paddy straw based nutrient broth. In solid state fermentation, maximum laccase, endoglucanase, exoglucanase and - glucosidase activities of 203 Ug-1, 35.7 Ug-1 , 14.2 Ug-1 and 11.3 Ug-1 respectively were obtained as compared to 11.2 Uml-1, 6.46 U/ml-1, 1.85 U/ml-1 and 2.13 U/ml in submerged conditions. The pretreatment of paddy straw with Delftia sp. PP4_S3 showed maximum reduction of 7.46% total solids, 5.67% volatile solids, 40.6%cellulose, 22.5% hemicellulose, 45.7% lignin and 17.7% silica in 5 days of pretreatment period. Highest biogas production i.e. 180 l/kg PS, 560 l/kg TS and 673.2 l/kg VS (66.1% enhanced over control) was found on 3rd day of treatment. Paddy straw pretreated with Coriolus versicolor MTCC 138 showed 44.7% increased biogas production with 153.4 l/kg PS, 561.9 l/kg TS and 689.9 l/kg VS. Pretreatment of paddy straw with Coriolus versicolor MTCC 138 showed maximum reduction of 3.71% total solids, 4.61% volatile solids, 38.6%cellulose, 14.8% hemicellulose, 42.6% lignin and 16.2% silica. Both Delftia sp. PP4_S3 and Coriolus versicolor MTCC 138 showed significant reduction in lignin and silica content which indicate that both are lignin and silica degrader. However less pretreatment period required by bacteria make it more suitable for biogas production.ThesisItem Restricted Characterization of phosphate solublizing bacteria from potato field soil for plant growth promotion activities(Punjab Agricultural University, Ludhiana, 2017) Harpreet Kaur; Gangwar, MadhuramaThe present study was undertaken with a view to isolate phosphate solubilizing bacteria (PSB) from potato field soil and evaluate their potential to produce plant growth promoting metabolites and increase the crop yield. Phosphate solubilizing bacteria (PSB) are frequently used as plant growth promoters and their use as inoculants increases P uptake by the plant and enhances crop yield. A total of 40 PSB isolates were obtained from the rhizospheric soil of potato. The functional potentialities of the isolates recorded were phosphate solubilization, siderophore and Indole acetic acid (IAA) production. The maximum P-solubilization and IAA production was recorded for isolate DS-20 (45.28 mg /100 ml and 80.12 µg/ml) respectively. Isolate DS-5 exhibited maximum catechol type (78.8µg/ml) and hydroxamate type (43.56 µg/ml) of siderophore production. A field experiment was conducted to evaluate the tuber yield and quality characteristics in response to three phosphorus levels (0, 75 and 100 kg/ha) and two PSB cultures (DS 20 and Reference culture). The crop growth (sprout number, plant height, leaf area, chlorophyll content) and yield traits (tuber yield, NPK contents of plant, tuber and soil) enhanced on PSB inoculation as well as increased P-fertilizer application. Maximum tuber yield of 375 q-1 hectare was produced by Reference culture. Therefore, PSB inoculants may act as potential P-biofertilizer for the sustainable potato cultivation system.ThesisItem Open Access Studies on fermentation parameters for production of industrial alcohol from broken and damaged rice grains(Punjab Agricultural University, 2011) Harpreet Kaur; K. L., KalraTwo different isolates of Aspergillus sp. procured from starchy substrate (rice grain flour) were screened for total amylase and glucoamylase activity. Among which Aspergillus oryzae (green) was selected for the further study as it yielded maximum total amylase (20.15 U/ml) and glucoamylase activity (5.2U/ml) as compared to Aspergillus niger (black). This Aspergillus oryzae (green) culture got accession no. HQ825090 after the identification at molecular level by NCBI. The cultural conditions optimized by Response Surface Methodology (RSM) according to Box-Behnken design comprising of five factors at three levels for maximum total amylase production and glucoamylase production. The optimized conditions for best enzyme production were found to be temperature 29.880C, pH 4.74, moisture content 65.88% incubation time 3.78 and inoculum concentration 9.91%v/w Further, liquefaction and saccharification of the rice grain powder was optimized by using varying amount of the crude amylase and 8ml of crude amylase in 20% substrate concentration was found to be best with maximum % saccharification about 79.95% after 6 hours of enzymatic hydrolysis. Further, for simultaneous saccharification and fermentation, different fermentation parameters were optimized by Response Surface Methodology (RSM) according to Box- Behnken design comprising of four factors at three levels for maximum bioethanol production by Pichea kudriavzevii (Issatchenkia orientalis) procured from CIPHET, Ludhiana identified at molecular level by NCBI and available in public domain with accession no. HQI22942. The maximum ethanol yield was found to be 69.25g/l in the Erlenmeyer flask fermentation. The optimized cultural conditions for bioethanol by RSM were temperature 34.08°C, pH of medium 4.8, aeration 126.13rpm and inoculum concentration 11.04%(v/v) containing 1x109 yeast cells/ml. Thus under optimized conditions scale up studies of bioethanol production was carried out in laboratory scale fermenter of 2.5L capacity and results showed an slight increase in the bioethanol yield of 71.68g/l with 90% fermentation efficiency as compare to 69.25g/l in the Erlenmeyer flask fermentation, as better controls in the fermenter throughout the experiment