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Subject: Microbiology × Author: Arashpreet Kaur × End date: 2019 × Start date: 2017 ×
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    Effect of storage on seed borne aflatoxin producing mycoflora in wheat, paddy and rice
    (Punjab Agricultural University, Ludhiana, 2017) Arashpreet Kaur; Arora, Maninder
    Wheat, paddy and rice are widely cultivated cereal crops in India. In the present study, the stored samples of wheat, paddy and rice were collected from SWC (State Warehousing Corporation) Rampura Phul, CWC (Central Warehousing Corporation) Moga and FSD (Food Supply Depot) Khanna under covered storage, FSD Moga under cover and plinth storage. The storage period was 18 months. The samples were analysed after every three months, for the total incidence of mycoflora, aflatoxin contamination and physico-chemical characteristics. The seed associated mycoflora was isolated by using standard blotter paper method. Percentage (%) incidence of seed borne fungi increased during the entire storage. Asergillus sp. was recorded at maximum level, followed by Rhizopus sp. and Penicillium sp. Aflatoxins were analysed qualitatively and quantitatively by using Pressure Mini Column method and Liquid Chromatographic method, respectively. The incidence of aflatoxin contamination increased during the storage. Aflatoxin contamination was found Below Detection Limit (Method Detection Limit = 5µg/kg) for all the samples, which showed positive results for aflatoxin contamination during qualitative assessment. During the entire storage, the moisture content of wheat, paddy and rice samples increased significantly (P < 0.05). The moisture content of all the samples was found to be less than 12 %. The level of protein, fat, fibre and ash content of these cereals decreased significantly (P < 0.05), while the decrease in carbohydrate content was non-significant. The incidence of seed borne mycoflora, aflatoxin contamination and nutritive losses were higher in Khanna, followed by that in Moga and Rampura Phul. The samples taken from CAP storage had higher incidence of mycoflora, aflatoxin contamination and nutritive losses as compared to that in the covered storage. Hence, covered storage was better for retaining the nutritive value of stored grains and maintaining the microbiological quality.