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20191
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Subject: Microbiology × Author: Anjali × End date: 2019 ×
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    Comparison of shelf life and bioefficacy of liquid and charcoal based biofertilizer in chickpea
    (Punjab Agricultural University, Ludhiana, 2019) Anjali; Sharma, Poonam
    The present investigation was carried out to compare the shelf life and bioefficacy of liquid and charcoal based biofertilizers of LGR33+RB1 amended with different additives viz. 0.1% CMC, 2% PVP and 10mM trehalose stored at 4⁰ and 28⁰C upto 300 days in chickpea. Viable cell count as well as plant growth promoting (PGP) traits viz. IAA production, P solubilization and siderophore production were obtained at an interval of 30 days and results revealed that all liquid based biofertilizer treatments with additives recorded significantly high viable population, IAA production and P solubilisation during the entire study period (300 days) at 4⁰ and 28⁰C as compared to charcoal based biofertilizer treatments. However, among different additives amendment with 0.1% CMC showed maximum viable population and PGP traits in both liquid as well as charcoal based biofertilizers. In vitro seed germination experiment was conducted to evaluate the effect of liquid and charcoal based biofertilizer amended with different additives on growth parameters in chickpea. Liquid biofertilizer treatment LGR33+RB1+0.1% CMC recorded significantly high plumule length (12.5cm), radical length (5.8cm), fresh weight and dry weight of seedling (0.72 and 0.68 g, respectively) along with improved seed survival rate of inoculants on chickpea seeds upto 48 hrs (8.27log cfu/ml). In vivo studies all liquid as well as charcoal based biofertilizer treatments with additives improved growth parameters in chickpea as compared to recommended consortium treatment without additives. Liquid biofertilizer treatment LGR33+RB1+0.1% CMC recorded maximum seed emergence, plant height, chlorophyll content, number of nodules, dry weight of nodules as well as leghaemoglobin content, soil enzyme activity, total N and P content of shoot and soil along with improvement in grain yield over uninoculated control.