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Subject: Microbiology × Author: Amanpreet Kaur × End date: 2019 × Start date: 2017 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Cumulative effect of neem powder, Azotobacter and indole-3-acetic acid (IAA) growth hormone on yield potential of Agaricus bisporus (Lange) Sing
    (Punjab Agricultural University, Ludhiana, 2017) Amanpreet Kaur; Sodhi, H.S.
    A. bisporus is the most popular cultivar among the artificially grown fungi in the world. The mushroom cultivation using agricultural crop lignocellulosic residues has been standardized but the supplementation accurately equilibrates the nitrogen content and the C/N ratio. Neem powder, Azotobacter and Indole-3-acetic acid were used as supplements at different stages of Agaricus bisporus U3 and S11strains. The neem powder was supplemented @ 0.5% to 2.0% in ready compost and spawned at @ 0.7%. The yield potential of Agaricus bisporus U3 was significantly increased upto 29.47% when neem powder was supplemented @ 0.5% and 1.0% at the time of spawning. Azotobacter was added in casing soil @50ml (107-108cfu/ml) in 5kg casing mixture along with neem powder. Use of Azotobacter in the casing had shown 16.0% increase with respect to control I (No addition) a further increase upto 19.41% in yield was recorded, when Azotobacter was applied on bags filled with compost supplemented with 0.5% and 1.0% neem powder respectively. IAA spray at the time of pinhead formation and at the time of 1st flush and 2nd flush showed no significant increase in yield with respect to control I (No addition). However, use of IAA on bags supplemented with 0.5% and 1.0% neem powder and Azotobacter at the time of pinhead formation indicated maximum yield potential of Agaricus bisporus U3. The observations made on quality parameters indicated 2.8 to 3.2 cm stipe length, 3.8 to 4.3cm pileus diameter, color 52.11 to 89.67 L-value and 5125to 11143 hardness was observed in Agaricus bisporus U3. In Agaricus bisporus S11 showed 56.23% increase in yield when neem powder was supplemented @1.0% at the time of spawning. Use of Azotobacter in the casing had shown 31.00% increase with respect to control I (No addition) a further increase of 17.46% in yield was recorded, when Azotobacter was applied on bags filled with compost supplemented with 1.0% neem powder. IAA spray at the time of pinhead formation and at the time of 1st flush and 2nd flush showed no significant increase in yield with respect to control I (No addition). However, use of IAA on bags supplemented with 0.5% and 1.0% neem powder, Azotobacter at the time of 1st flush and 2nd flush indicated maximum yield potential of A. bisporus S11. While in Agaricus bisporus S11 the observations made on stipe length, pileus diameter, color and hardness indicated 2.8 to 3.2cm stipe length, 2.9 to 4.3cm pileus diameter, color 48.3 to 75.9 L-value and 4120 to10364 hardness was observed.