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Subject: Microbiology × Author: Amandeep Kaur × End date: 2022 ×
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    PRODUCTION OF Beauveria bassiana (BALSAMO) VUILLEMIN USING AGRICULTURAL PRODUCTS
    (Punjab Agricultural University, Ludhiana, 2013) Amandeep Kaur
    Beauveria bassiana isolates were mass produced on different solid substrates and effect of carrier and viability of formulation at different storage temperatures were studied. The effect of insecticides on B. bassiana growth at concentrations (one fourth MC, half MC and MC) was also studied. Seven solid substrates were evaluated for growth parameter of four Beauveria isolates and variability of the mean conidial count and colony forming unit was recorded among substrates and isolates. Among B. bassiana isolates, maximum mean conidial count (25.8×105 conidia/g) and colony forming unit (22.8×105cfu/g) was recorded in B. bassiana MTCC 6291. Among solid substrates, rice recorded maximum mean conidial count (31.8×105conidia/g) and colony forming unit (28.6×105cfu/g). Three Beauveria which were mass cultured on rice, sorghum and maize were formulated with talc, charcoal and oil as carriers. Among all the formulations maximum cfu count (44.6×105cfu/g) was recorded in talc based formulation of B. bassiana MTCC 6291 on rice. It was found that minimum per cent decrease (7.3% and 7.6% ) of viable count was recorded at 4°C in talc based formulation of B. bassiana MTCC 6291 and B. bassiana MTCC 2028, respectively. Further, to study the effect of insecticides on Beauveria growth at different concentrations, radial growth of Beauveria varied among strains. It was conclude that in all insecticides tested, radial growth varied in inverse proportion to the concentration. Lower the concentration more the radial growth recorded. Among all the insecticides tested, quinalphos was least compatible with B. bassiana strains and showed maximum mean growth percent inhibition over control (58.5%) while it was minimum in triazophos (34%).