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  • ThesisItemOpen Access
    Effect of planting densities and training sysems on light interception, growth, productivity and nutrient composition of peach
    (Department of Horticulture College of Agriculture PAU, Ludhiana, 2001) Singh, Harminder; Kanwar, J. S
  • ThesisItemRestricted
    EFFECT OF PUTRESCINE AND CALCIUM CHLORIDE ON THE STORAGE AND QUALITY OF MANGO (Mangifera indica) cv. DUSEHRI
    (PAU Ludhiana, 2011) Madhu Kumari; Harminder Kaur
    The investigations on the “Effect of putrescine and calcium chloride on the storage and quality of mango cv.Dusehri” were conducted in the Department of Horticulture, PAU, Ludhiana during 2009-2010. In group 1 experiment, mango trees were sprayed with putrescine (1.0, 2.0, 3.0 mmol/l) and calcium chloride (1.0, 2.0, 3.0%) solution 10 days before harvest. Fully matured fruits were harvested, washed, disinfected, dried and packed in corrugated fibre board boxes (CFB). In group II experiment, fully matured fruits were dipped in putrescine (1.0, 2.0, 3.0 mmol/l) and calcium chloride (1.0, 2.0, 3.0%) solutions. Thereafter, fruits were dried and packed in corrugated fibre board boxes. Packed fruits from both the groups were stored for 20 days in cold room (13±10C temperature) and 85-90% R.H. Physico chemical analysis of fruits were done after 5, 10, 15 and 20 days of storage. General appearance of fruits was better in treated fruits as compared to highly shrivelled control fruits in both the groups. Treatment of mango fruits with chemicals significantly reduced the physiological fruit weight loss. The maximum reduction in fruit weight loss was recorded in putrescine 2.0 mmol/l and CaCl2 2.0 per cent in both groups. Rotting of fruits was better checked with putrescine and calcium chloride treatments. Minimum rotting percentage was recorded in putrescine 2.0 mmol/l and CaCl2 2.0 per cent. Organoleptic rating was better with treatment CaCl2 2.0 per cent and putrescine 2.0 mmol/l in both the group. Total soluble solids, TSS/acid ratio, -carotene content total and reducing sugars increased as the storage period advanced and were higher in control fruits. The acidity of fruits decreased as the storage period increased and maximum decrease in acidity was observed in control fruits. pre harvest spray of putrescine 2.0 mmol/l (T8), calcium chloride 2.0 per cent (T11) and post harvest dip of putrescine 2.0 mmol/l and calcium chloride 2.0 per cent were the best treatments for prolonging the shelf life of mango fruits, by reducing the physiological loss in weight and rotting , delaying ripening and maintaining the fruit quality during storage at 13 ±10C temperature. However post harvest dip of calcium chloride 2.0 per cent was found more effective and economical than pre harvest spray.
  • ThesisItemRestricted
    “Studies on improvement of fruit quality through girdling and thinning in Satluj Purple plum”
    (PAU Ludhiana, 2011) Neeraj Sharma; Harminder Kaur
    The present investigation entitled “Studies on improvement of fruit quality through girdling and thinning in Satluj Purple plum” was carried out in the New Orchard, Department of Horticulture, Punjab Agricultural University, Ludhiana during 2009. Plants of Satluj Purple plum planted at 3x1.5 m were used as experimental material. In experiment I, Trunk and limb girdling was done at full bloom and 7,14,21,28 and 35 days after full bloom. All the girdling treatments advanced fruit maturity as compared to control. Least number of days taken for maturity were recorded when limb girdling was done 21 days after full bloom. Yield was found to increase and fruit drop was reduced in all the treatments. Maximum yield, fruit weight and size, pulp/stone ratio, colour and better fruit quality in terms of higher TSS, TSS/acid ratio, β-carotene with lower acidity and firmness were observed when limb girdling was done 21 days after full bloom. Healing of the girdle was satisfactory and there was no detrimental effect noticed in any of the treatments. Leaf nitrogen and chlorophyll content were decreased and total carbohydrates were increased with the girdling. In experiment II, Hand thinning was done at fruit set and 7, 14,21and 28 days after fruit set. Hand thinning treatments advanced fruit maturity and also resulted better quality of fruits as compared to control. Higher yield fruit weight, size, colour, TSS, TSS/acid ratio and lower acidity and minimum firmness were recorded when hand thinning was done 14 days after fruit set. Significant increase in β-carotene and diameter of thinned fruits was recorded with hand thinning as compared to control. Leaf nitrogen and chlorophyll content were found to decreased whereas total carbohydrates increase with hand thinning treatments.
  • ThesisItemOpen Access
    MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF GERMPLASM AND F1 HYBRIDS.
    (PAU Ludhiana, 2013) Daljinder Singh; M. I. S, . Gill
    The present investigation entitled “Morphological and molecular characterization of guava (Psidium guajava L.) germplasm and F1 hybrids” was conducted during years 2010 to 2013 at P.A.U., Ludhiana and Regional Fruit Research Station, Bahadurgarh. Thirty five guava genotypes grafted on seedling rootstocks were evaluated for tree, vegetative, reproductive, fruit and seed characters based on UPOV descriptors. Statistically significant difference for all the traits was observed among tested genotypes. Among components of genetic variation, phenotypic and genotypic coefficient of variation for different traits ranged from 7.26 and 6.95 to 35.00 and 33.11 %, respectively. Divergence analysis based on D2 statistic grouped the tested genotypes into six clusters with variable number of genotypes in each cluster, indicating the presence of genetic diversity in them. Cluster I, cluster IV, cluster V and cluster VI showed intra cluster distance of 55.12, 55.40, 50.70 and 61.84 respectively, indicating sufficient dissimilarity among genotypes for morphological features. Molecular characterization using 80 SSR primer pairs was done enabling cluster analysis of 43 guava genotypes. Among the primers, 78 markers amplified 258 alleles across the 43 genotypes with an average of 3.25 alleles per locus. The highest number of seven alleles per locus was amplified by mPgCIR 32, followed by six alleles per locus by mPgCIR98. The power of discrimination was found to be least (0.00) for markers mPgCIR251 and mPgCIR 414 and highest (0.84) for markers mPgCIR137. Values of expected heterozygosity ranged from 0.00 in monomorphic locus (mPgCIR251 and 414) to maximum 0.79 for locus mPgCIR32, with an average 0.47 expected heterozygosity. Estimated genetic dissimilarity coefficient ranged from minimum 0.020 (between genotypes 14-10 and 12-11) to 0.590 (between One Kg and Portugal) with an average dissimilarity of 0.322 across all the genotypes. Cluster analysis on the basis of molecular analysis classified the guava genotypes into two major groups having 3 and 40 genotypes each. The dendrogram generated using SSR markers along with the phenotypic data will be useful for choosing the diverse parents for further guava breeding programmes. A total of nine F1 hybrids were generated and vegetative parameters were recorded in different hybrids to distinguish them, as well as SSR markers were also used to confirm the parentage of these hybrids.
  • ThesisItemRestricted
    EFFECT OF CHEMICALS ON STORAGE LIFE AND QUALITY OF BER CV. UMRAN
    (PAU Ludhiana, 2011) Salveen Kaur; JS, Bal
    The investigations on “Effect of chemicals on storage life and quality of ber cv. Umran” was conducted in the Department of Horticulture, PAU, Ludhiana during year 2011. The fruits were harvested at peak maturity period (29th March) along with pedicel and were dipped in ascorbic acid, boric acid and sodium carbonate (1, 2, 3 %) each for 5 min. Treated fruits were packed in Corrugated Fibre Board boxes of 2 Kg capacity and kept in cold chamber at 7ºC temperature and 90-95 per cent RH. The fruits were analysed after 7, 14, 21 and 28 days of storage. The lowest physiological loss in weight was found in ber fruits treated with ascorbic acid 2 per cent and sodium carbonate 1 per cent. High palatability rating was noted in fruits dipped in ascorbic acid 1-3 per cent. The spoilage of fruits was noted significantly less in fruits treated with ascorbic acid treatments. The highest total soluble solids and ‘b’ value of fruit colour was recorded in the fruits dipped in boric acid 3 per cent. The highest mean fruit firmness, pulp/stone ratio and total sugars were noted in the fruits treated with ascorbic acid 3 per cent. The pedicels were properly retained with fruits up to 21 days of storage. The lowest acid content was estimated with boric acid 3 per cent and sodium carbonate 1-2 per cent. Higher retention of vitamin C was observed in fruits with ascorbic acid 2 and 3 per cent treatments. The physiological loss in weight, spoilage and TSS/ acid ratio were increased with prolongation of storage days whereas palatability rating, fruit firmness, pulp/stone ratio, acidity, vitamin C were decreased with advancement of storage period. TSS and total sugars increased significantly up to 14 days of storage but followed a decrease at later period of storage. It was concluded that Umran ber kept with pedicel could be stored satisfactorily for 21 days in cold chamber (7ºC) without any major effect on their quality.
  • ThesisItemOpen Access
    Effect Of Foliar Application Of Potassium On Yield And Quality Of Kinnow
    (Punjab Agricultural University ;Ludhiana, 2002) Parmpal Singh; Singh, S. N.
  • ThesisItemOpen Access
    Vegetative And Fruiting Behaviour Of Semi-Soft Pear Strains In Relation To Nutrient Status
    (Punjab Agricultural University; Ludhiana, 2002) Tajinder Singh; Sandhu, A. S.
  • ThesisItemRestricted
    Effect Of Plant Bioregulators On Seediness And Fruit Quality Of Winter Crop Of Guava (Psidium Guajava L.).
    (Punjab Agricultural University ;Ludhiana, 2003) Balwinderjit Kaur; kanwar, J. S.
  • ThesisItemOpen Access
    Assessment of genetic divergence and hybridization studies in pomegranate germplasm
    (PAU, 2013) Raina, Dimpy; Gill, P.P.S.
    The present investigation entitled ―Assessment of genetic divergence and hybridization studies in pomegranate germplasm‖. The objectives of this study were to assess genetic diversity based on horticultural traits, characterization of pomegranate genotypes using DNA markers, effectiveness of pollen storage conditions and exploration of the possibilities of F 1 hybrid production during year 2011-12. A significant and wide range of variation was observed among genotypes for various quantitative characters. Higher coefficient of variation was observed for characters like number of hermophrodite flowers per tree (26.67), number of fruits per tree (20.55), yield per tree (22.73), acidity (11.62) and TSS/acid ratio (13.23). ‗Ganesh‘ recorded maximum yield (21.16 kg/plant), fruit weight (309.3 g), aril weight (31.62 g /100 aril weight), peel weight (103.42 g) and TSS (13.39 %) but with minimum juice per cent (28.54 %). Mridula was observed promising for fruit length (6.75 cm), fruit breadth (7.85 cm), TSS/Acid ratio (51.18) and less in acidity (0.26 %). Highest juice per cent (67.26) and lowest TSS (11.0 %) was found in ‗Anar Shirin‘. Jhodpur White had highest number of hermophrodite flowers (338.3) and fruits per tree (60.84). The yield per tree was found correlated positively with fruit weight, aril weight and peel weight and negatively with juice per cent. The clustering of genotypes into eight different clusters was based on mean values of quantitative characters. The maximum inter -cluster distance of 72.74 was observed among genotypes of the cluster V and VII and minimum (23 .85) between the cluster IV and III. The principal component analysis showed that more than 82 per cent of the variability observed for quantitative characters in different pomegranate genotypes. Genetic divergence among the genotypes was estimated by 47 SSR markers. Six SSR markers (Pom010, ABRII-MP28, PGCT046, PGCT088, PGCT112 and PGCT037) showed monomorphic pattern and 41 showed polymorphic patterns with amplification of alleles ranging from 2 to 4. PIC value ranged from 0 and 0.66 (PGCT093) among 41 polymorphic primers. The UPGMA clustering grouped the genotypes into three main clusters I, II and III. The cluster I comprised of one genotype followed by the cluster II which contained eight genotypes, whereas, sub-cluster IIIA contained 12 genotypes, five and four in sub-cluster IIIB and IIIC, respectively. Genetic similarity values between genotypes ranged from 0.78 to 0.95 and dissimilarity was only 0.17. Maximum pollen viability was observed at -200 C storage temperature for 9 weeks and highest viable pollens found in Ganesh (95 %) followed by ‗Mridula‘ (94.7 %), ‗Jyoti‘ (92.7 %) and ‗Kandhari‘ (92 %). Highest pollen germination was recorded in Ganesh (78.9 %) followed by ‗Mridula‘ (75.8 %), Jyoti (58.9 %) and minimum in ‗Kandhari‘ (51.6 %). In hybridization study of pomegranate genotypes 80.47 per cent success rate in terms of fruit set after crossing was achieved. Highest fruit set observed in ‗Mridula‘ x ‗Ganesh‘ (85.54%) and lowest in ‗Mridula‘ x ‗Kandhari‘ (76.61 %). Five SSR primer pair (PGCT093, PGCT059, PGCT097, PGCT111, and ABRII-MP42) was found to produce the polymorphic alleles to confirm the hybridity of hybrids.