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ThesisItem Open Access Light Interception Behaviour Of Guava And Its Effects On Vegetable Growth, Fruit Yield And Quality(Punjab Agricultural University ;Ludhiana, 2003) Ajitpal Singh; Dhaliwal, G. S.ThesisItem Open Access STUDY ON GENETIC DIVERSITY IN GRAPES USING MORPHOLOGICAL TRAITS AND DNA MARKERS(PUNJAB AGRICULTURAL UNIVERSITY, 2010) Ajitpal Singh; Gill, M.I.SThe present investigation was conducted on “Study on genetic diversity in grapes using morphological traits and DNA markers”. The objective of this study was to estimate the diversity among 26 grape accessions maintained at New Orchard of Punjab Agricultural University. Genetic diversity among accessions was estimated using morphological traits and SSR marker data. 30 primer pairs resulted in amplification of 1201 putative alleles in 26 genotypes with an average of 46.19 alleles per genotype. PIC values ranged from as low as 0.47 to as high as 0.59 with an average of 0.38 for all 30 primers. On basis of SSR data, dendrogram clustered 26 genotypes into two major groups. The lowest similarity coefficient (0.50) was shown between major group I consisting of all cultivated varieties and hybrids and major group II consisting of all the rootstocks. The dendrogram based on SSR markers showed no congruence with morphological data. The dendrogram of 30 genotypes was constructed based on ten morphological markers. The genotypes were divided into five groups with genetic similarity values ranging from 0.46 to 0.90. The diversity analysis using morphological markers was not in agreement with pedigree of 30 genotypes. Using molecular markers data the grape germplasm was clearly subdivided into cultivars and rootstocks indicating that for assessing the genetic diversity, molecular markers were more reliable and authenticated. Key Words: Genetic diversity, Grapes (Vitis vinifera),, morphological traits
