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Subject: Genetics and Plant Breeding × Author: Bhupinder Singh × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: M.Sc ×
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    Introgression of cotton leaf curl disease resistance from Gossypium armourianum to G. hirsutum
    (Punjab Agricultural University, Ludhiana, 2018) Bhupinder Singh; Rathore, Pankaj
    The present investigation was undertaken to evaluate the performance of interspecific F1 hybrid and backcross derivatives of G. hirsutum and G. armourianum cross for response to cotton leaf curl disease as well as to identify the alien segments in the backcross derivatives. Experimental plant material consisted of G. armourianum Acc. PAU 1, G. hirsutum cv. F 1861 and LH 1556, their F1, BC1F1, BC2F1 derivatives. The interspecific F1hybrid between G. hirsutum and G. armourianum was found to be completely resistant to CLCuD. Two of the four BC1F1 plants were observed to be CLCuD resistant. Out of 84 BC2F1 plants, 43 were observed to be resistant, whereas 41 plants were susceptible to CLCuD. Total genomic DNA of the experimental plant materials was amplified employing virus specific primer pairs. Presence of virus DNA was detected in the American cotton parents as well as CLCuD susceptible backcross derivatives. Of the 100 SSR markers used to detect polymorphism in the parental lines, 52 were polymorphic and were used to identify alien segments in CLCuD resistant BC1F1 Plant 2. Eight markers were amplified in this plant demonstrating introgression from the G. armourianum parent. These primers were used to genotype 84 BC2F1plants. The range of G. armourianum specific alleles in the BC2F1plants was observed to be 38.09 through 75.0%. The introgression of G. armourianum specific chromosomal segments in the BC2F1 plants as revealed by SSR markers was independent of the CLCuD response suggesting that none of the eight primers may be linked to CLCuD resistance. Markers TMB 1271 and NAU 6316 exhibited segregation distortion and were transmitted in higher frequency than expected in the BC2F1 progeny.