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Subject: Genetics and Plant Breeding × Author: Batra, Ritika × End date: 2019 × Start date: 2014 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Anatomical and genetic analysis of damaged grains in rice (Oryza sativa L.)
    (Punjab Agricultural University, Ludhiana, 2019) Batra, Ritika; Mangat, Gurjit Singh
    Rice grain discoloration or rice black pinhead damage reduces the yield of milled rice and directly reflects on consumer acceptability. The black pinhead damage in grains of PR125 and F3 population of cross PR121 X PR125 and PR121 X PR126 were studied for its percentage damage intensity, anatomical evaluation and marker analysis. The range of percentage damage intensity for both the crosses was 0.5-7.2 and 0.5- 4.9 per cent respectively. The anatomical evaluation was done using scanning electron microscopy (SEM) and the results showed that there was malformation of starch granules at cellular level. The starch granules of damaged grains were loosely packed while they were densely packed in undamaged ones. 200 SSR markers were applied to check parental polymorphism and 15% (30 out of 200) polymorphism was recorded between parents. Two bulks were prepared for bulked segregant analysis, in which one was with maximum percentage damage and other with no damage. From the above 29 polymorphic markers, only 10% polymorphism was observed in the bulks. Chi square analysis was done to check the inheritance of black pin head damage. Since the statistical analysis followed normal distribution, the damage was attributed to be controlled by two or more genes.