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  • ThesisItemOpen Access
    Association mapping for Ascochyta blight, Botrytis grey mould and yield contributing traits in a Reference Set of chickpea (Cicer arietinum L.)
    (Punjab Agricultural University, Ludhiana-, 2020) Aiswarya S. Kumar; Sarvjeet Singh
    Evaluation and exploitation of genetic diversity is crucial for crop improvement programmes for the efficient utilization of potential genetic sources for desirable traits. The chickpea Reference Set comprising 288 accessions used in this study, constituted by ICRISAT, Patancheru, represents the diverse global composite collection of chickpea and is a reliable source for diversity. The present study was conducted at the Experimental Farm and Laboratories of Pulses Section, Dept. of Plant Breeding & Genetics, PAU, Ludhiana, Punjab during rabi seasons of 2018-19 and 2019-20. The experimental material was evaluated for resistance against Ascochyta blight and Botrytis grey mould diseases, yield and yield contributing traits to identify potential donors and identify associated QTLs under north Indian conditions using association mapping. The yield and contributing traits were evaluated under two phosphorus levels; Low-P (no phosphorus application) and High-P (40 kg/ha P2O5) for two consecutive years in Alpha Lattice design using two replications. One genotype ICC 1915 having high level of resistance to Ascochyta blight and one genotype ICC 6579 having moderate level of resistance to Botrytis grey mould were identified as promising donors for respective diseases. Days to 50% flowering, plant height, number of pods, seeds per pod, 100-seed weight and biological yield showed high to moderate levels of broad sense heritability and genetic advance, implying high response to selection. The traits namely number of secondary branches, number of pods, biological yield and seed yield can be effectively used for selection under low-P regime. Significant and positive correlations were observed between seed yield and these contributing traits. Genotypes namely ICC 5639, ICC 7413, ICC 11584, ICC 13357, ICC 9590, ICC 2072 and ICC 4418 were identified as phosphorus use efficient based on their better performance for yield and yield contributing traits under Low-P compared to high-P conditions. However, association studies failed to establish any Marker Trait Associations (MTAs) for disease resistance and yield contributing traits.