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ThesisItem Restricted Generation of linkage map and development of new hybrids for improved fruit colour in guava (Psidium guajava L.)(Punjab Agricultural University, Ludhiana, 2017) Harjot Singh; Gill, M.I.S.The present study entitled “Generation of linkage map and development of new hybrids for improved fruit colour in guava (Psidium guajava L.)” was carried out during the years 2014 to 2016. The existing, eight F1 hybrids developed as a result of cross combinations involving Allahabad Safeda, CISH G-1, CISH G-4 (Shweta), Safri as female parents and CISH G-1, 1716, Malaysian guava, Allahabad Safeda, L-49 (Sardar Guava) as male parents were studied for their morphological and fruit physio-chemical characters. The new F1 hybrids were developed out of a cross combination Allahabad Safeda × Purple Guava, Allahabad Safeda × CISH G-1 and Allahabad Safeda × Seedless with the aim to improve fruit colour and their morphological characters were recorded. The existing hybrids and new hybrids were developed and maintained at Fruit Research Farm, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Morphological characters of guava hybrids were recorded on basis of UPOV descriptors. Vegetative characters were observed during growing season, followed by fruit characters. Among eight hybrids; the hybrid Allahabad Safeda × CISH G-1 had sub-globose fruits with deep pink flesh, maximum percent Total Soluble Solids (12.82), Juiciness (54.27) and anthocyanin reflective index (0.210). Intensity of anthocyanin was recorded maximum (0.266) in Purple Guava and minimum (0.014) in Allahabad Safeda. While, in the progeny of the cross Allahabad Safeda × Purple Guava, the anthocyanin intensity (0.214) was close to that of Purple Guava. A total of 30 polymorphic SSR markers were amplified on the 238 segregating F1 progeny plants of Allahabad Safeda × Purple Guava and 29 SSRs were mapped on 10 linkage groups with total map size of 345.63 cM. Linkage map was constructed by using MAPDISTO. A STS marker MdMYB10, linked to apple anthocyanin candidate gene was found polymorphic and it showed linkage with leaf anthocyanin content in guava also. Further enrichment of the linkage map will lead to the development of new markers for mapping traits of economic importance.