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    Diversity analysis and characterization of morpho- anatomical and photosynthetic traits in mandarin (Citrus reticulata Blanco)
    (Punjab Agricultural University, Ludhiana, 2019) Arshdeep Singh; Uppal, Gurteg Singh
    The present study entitled “Diversity analysis and characterization of morpho- anatomical and photosynthetic traits in mandarin (Citrus reticulata Blanco)” was carried out during the years 2017-18 and 2018-19 at Punjab Agricultural University, Ludhiana. Fifteen mandarin genotypes grafted on rough lemon (Citrus jambhiri Lush) rootstocks were evaluated for morphological characters based on IPGRI descriptors. Furthermore, the leaf samples of these genotypes were imaged in scanning electron imaging mode in (model Hitachi S-3400 N) SEM for morpho-anatomical and photosynthetic diversity analysis. The genetic diversity based on these traits was assessed by using DARwin software. The dendrogram grouped genotypes into six different clusters based on morphological characters. The highest similarity coefficient (0.861) was recorded in genotypes Dancy and Wilking. However, Michal showed lowest (0.402-0.539) genetic similarity coefficient with all other mandarin genotypes and these were genetically distinct from each other. The Genotypic Variance (VG) and Phenotypic Variance (VP) of morphological characters were maximum for fruit weight followed by rootstock girth, fruit granulation, scion girth and pollen viability. The heritability was maximum for traits like seed weight, aerola diameter, pedicel length, length of filament, length of style, petal length and vesicle length. Similarly, dendrogram grouped genotypes into five different clusters based on morpho-anatomical and photosynthetic characters. The Genotypic variance (VG) and Phenotypic Variance (VP) were maximum for stomatal density (4531.37 and 7385.39, respectively) followed by number of closed stomata, number of open stomata and palisade parenchyma tissue length. The maximum genetic advance was recorded for stomatal density (108.62). PAU Kinnow 1, a mutant of Kinnow was clubbed in separate cluster due to variation with respect to morpho-anatomical traits. This clearly differentiated the Kinnow from its mutant PAU Kinnow 1. The study clearly indicated presence of moderate level of genetic diversity within mandarin germplasm and this can be exploited for future mandarin breeding programme.