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  • ThesisItemOpen Access
    Uptake and metabolism of imidacloprid in cotton (Gossypium hirsutum Linn.) and its persistence in soil
    (PAU, 2015) Sahoo, Sanjay Kumar; Singh, Balwinder
    The uptake and metabolism of imidacloprid in cotton was studied by following its application as seed treatment and foliar application. Samples of cotton were analyzed by High Performance Liquid hromatograph. Maximum residues of imidacloprid and its metabolites were found to be 1.52 and 5.23 mg kg-1 in cotton leaves collected 15 days after sowing the seed treated with imidacloprid @ 3.5 and 14 g a.i. kg-1. The initial deposits of total imidacloprid were found to be 2.05 and 6.19 mg kg-1 in leaves collected 0 day after its application @ 20 and 80 g a.i. ha-1, respectively. For the corresponding dosages, the residues of imidacloprid in cotton leaves were declined to below the determination limit of 0.01 mg kg-1 collected 45 and 60 days after sowing the treated seed and 21 and 45 days after foliar application. The samples of lint, cotton seed oil and seed cake did not reveal the presence of imidacloprid residues at harvest for both the cases. In soil samples, total residues of imidacloprid were found to be 1.59 and 3.39 mg kg-1 collected after 7 day of sowing the treated seed @ 3.5 and 14 g a.i. kg-1 respectively. These residues could not be detected 60 days after sowing for both dosages. Following foliar application of imidacloprid @ 20 and 80 g a.i. ha-1, the total residues of imidacloprid were found to be 0.48 and 1.42 mg kg-1 in soil samples collected on the same day. The residues dissipated to below the determination limit after 15 and 30 days of application. Based on enzyme activity studies, it has been found that imidacloprid at the recommended dosage did not show any deleterious effect on dehydrogenase, phosphatase and urease activities.