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Fractionation of bay leaf oil and its bioefficacy against root knot nematode Meloidogyne incognita
(Punjab Agricultural University, Ludhiana, 2017) Mandeep Kaur; Chahal, K.K.
The present investigation deals with Fractionation of bay leaf oil and its bioefficacy against root knot nematode Meloidogyne incognita. The bay leaf essential oil was extracted by hydrodistillation using Clevenger apparatus. GC-MS analysis showed the presence of twenty one compounds. Eugenol, α-Pinene, isoeugenol, caryophyllene and 1,8-Cineole were the major compounds present in bay leaf essential oil. The essential oil was fractionated into its non-polar and polar fractions by column chromatography. α-Pinene, 1,8-Cineole and eugenol were isolated from bay leaf oil. Campholenic aldehyde was prepared from α-pinene and eugenol epoxide was prepared from eugenol using different reagents. The compounds isolated and derivatized were identified using FT-IR, 1HNMR and 13CNMR spectroscopy. The nematicidal activity of bay leaf oil, its fractions, compounds isolated and derivatized was evaluated against Meloidogyne incognita at different concentrations viz.0.20, 0.40, 0.60, 0.80, 1.00, 1.25 and 1.50 mg ml-1 for egg hatch inhibition and juvenile mortality studies. Bay leaf oil, polar fraction and eugenol were found to be very active against. M. incognita whereas αPinene and non-polar fraction showed less activity